Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,936,174	G→T	G47G (GGC→GGA)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,936,174	0	G	T	100.0%	97.7 / NA	28	G47G (GGC→GGA)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/14);  total (14/14)

TTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACG  >  NC_000913/1936099‑1936248                                                                            |                                                                           ttctttcATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGa                                                                        >  2:345497/1‑80 (MQ=255)    tttcATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAAt                                                                    >  2:345518/1‑81 (MQ=255)       cATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCg                                                                 >  1:345515/1‑81 (MQ=255)           aaaGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGgtg                                                             >  2:345522/1‑81 (MQ=255)             aGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTc                                                           >  1:345521/1‑81 (MQ=255)                 tCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCggg                                                       >  2:345520/1‑81 (MQ=255)                 tCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCgg                                                        <  2:345474/80‑1 (MQ=255)                   gAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCTCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGtt                                                     >  1:345513/1‑81 (MQ=255)                   gAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGtt                                                     >  2:345508/1‑81 (MQ=255)                   gAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGt                                                      <  1:345475/80‑1 (MQ=255)                    aGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGtt                                                     >  1:345511/1‑80 (MQ=255)                         cTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTg                                               <  1:345476/81‑1 (MQ=255)                            gcgGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGAcc                                            <  1:345477/81‑1 (MQ=255)                               gACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGc                                         <  1:345478/81‑1 (MQ=255)                                aCAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCt                                        <  2:345479/81‑1 (MQ=255)                                      ttGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                                   >  1:345517/1‑80 (MQ=255)                                      ttGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTc                                   >  2:345519/1‑80 (MQ=255)                                         gTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAg                                <  2:345481/80‑1 (MQ=255)                                         gTATATGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAg                                <  1:345480/80‑1 (MQ=255)                                           atatGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGtt                              >  2:345523/1‑80 (MQ=255)                                              tGCCGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATa                          <  1:345482/81‑1 (MQ=255)                                                 cGCTTTATCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAg                       <  1:345483/81‑1 (MQ=255)                                                       aTCCCAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAgg                 >  2:345525/1‑81 (MQ=255)                                                        tCCCAGTCAGCACGCCCTACTCCGATAATCCAGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGgc                <  2:345484/81‑1 (MQ=255)                                                          ccAGTCAGCACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGgcag              >  1:345526/1‑81 (MQ=255)                                                                  cACGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTac      <  1:345485/81‑1 (MQ=255)                                                                   aCGCCCTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTacg     <  2:345486/81‑1 (MQ=255)                                                                      cccTACTCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTacgacg  <  2:345487/81‑1 (MQ=255)                                                                            |                                                                           TTCTTTCATGAAAGTTTCGAGCGCCTCGCGGACAACTTTGGTATATGCCGCTTTATCCCAGTCAGCACGCCCTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACG  >  NC_000913/1936099‑1936248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑