| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,945,629 | A→G | I116I (ATT→ATC) | ruvA ← | Holliday junction branch migration complex subunit RuvA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,945,629 | 0 | A | G | 94.7% | 53.9 / ‑3.6 | 19 | I116I (ATT→ATC) | ruvA | Holliday junction branch migration complex subunit RuvA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (7/11); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
AAGAGATCGCCATGCAAACCTTTAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCAATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACGGCATTAACGAACTGCTGCGCTGACAT > NC_000913/1945558‑1945700 | aaGAGATCGCCATGCAAACCTTTAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGc < 2:347055/81‑1 (MQ=255) aTCGCCATGCAAACCTTTAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGttt > 2:347086/1‑81 (MQ=255) tCGCCATGCAAACCTTTAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTc < 1:347056/81‑1 (MQ=255) cAAACCTTTAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGc > 2:347085/1‑81 (MQ=255) ttAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCAct > 1:347090/1‑81 (MQ=255) tAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCAc < 1:347057/79‑1 (MQ=255) aTCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCActtctt > 1:347084/1‑81 (MQ=255) tCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCAATACCCGGCAGTTTCACCAGTGCCCCCACttcttc < 1:347058/81‑1 (MQ=255) cTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCt > 1:347091/1‑81 (MQ=255) tcatttcaACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTc < 2:347059/79‑1 (MQ=255) tcatttcaACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCaa > 1:347083/1‑81 (MQ=255) catttcaACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCaa < 1:347060/80‑1 (MQ=255) atttcaACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAAc < 1:347061/80‑1 (MQ=255) atttcaACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACg < 1:347063/81‑1 (MQ=255) atttcaACAATCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACg < 2:347062/81‑1 (MQ=255) aaTCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACGGCATTAAc > 1:347089/1‑81 (MQ=255) aaTCAAGCGTTCGGCGGTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACGGCATTAAc < 2:347064/81‑1 (MQ=255) gTTTTTTTGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACGGCATTAACGAACTGCTGCGCTGAc < 2:347065/81‑1 (MQ=255) tttttGCCGATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACGGCATTAACGAACTGCTGCGCTGACAt < 1:347066/80‑1 (MQ=255) | AAGAGATCGCCATGCAAACCTTTAAATCGGTCTTTCATTTCAACAATCAAGCGTTCGGCGGTTTTTTTGCCAATACCCGGCAGTTTCACCAGTGCCCCCACTTCTTCACGCTCAACGGCATTAACGAACTGCTGCGCTGACAT > NC_000913/1945558‑1945700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |