Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,962,324	T→C	V118A (GTT→GCT)	yecT →	protein YecT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,962,324	0	T	C	100.0%	54.9 / NA	17	V118A (GTT→GCT)	yecT	protein YecT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/10);  total (7/10)

CTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCG  >  NC_000913/1962251‑1962399                                                                          |                                                                            cTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGa                                                                       <  2:349747/80‑1 (MQ=255)   tAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGAtt                                                                     >  1:349766/1‑80 (MQ=255)              aGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCtt                                                         <  1:349748/81‑1 (MQ=255)                cGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTAt                                                       <  2:349749/81‑1 (MQ=255)                 gAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTAtt                                                      <  2:349750/81‑1 (MQ=255)                  aGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTc                                                     >  2:349777/1‑81 (MQ=255)                    ccTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTg                                                   <  1:349751/81‑1 (MQ=255)                           ggATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAg                                             >  1:349774/1‑80 (MQ=255)                           ggATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAg                                             >  2:349772/1‑80 (MQ=255)                             aTTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAAt                                          <  2:349752/81‑1 (MQ=255)                              ttttAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATg                                         <  1:349753/81‑1 (MQ=255)                                 tAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGc                                       <  1:349754/80‑1 (MQ=255)                                    ggACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCtctc                                   <  2:349755/81‑1 (MQ=255)                                                       aTCCTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGAc                 >  2:349779/1‑80 (MQ=255)                                                          cTTTCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCaa              >  1:349775/1‑80 (MQ=255)                                                             tCAAATGAGGACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATa            <  1:349756/79‑1 (MQ=255)                                                                     ggACGCTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCg  >  1:349782/1‑81 (MQ=255)                                                                          |                                                                            CTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCG  >  NC_000913/1962251‑1962399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑