Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,977,328	A→G	S173P (TCC→CCC)	flhC ←	DNA‑binding transcriptional dual regulator FlhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,977,328	0	A	G	100.0%	44.5 / NA	14	S173P (TCC→CCC)	flhC	DNA‑binding transcriptional dual regulator FlhC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/6);  total (8/6)

ACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGCAAAGCTGCCAACAGGCTG  >  NC_000913/1977256‑1977397                                                                         |                                                                      aCCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTAc                                                               >  2:352425/1‑81 (MQ=255)   cGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTACGt                                                             >  2:352436/1‑81 (MQ=255)      aTCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTACGTCtt                                                          >  1:352437/1‑81 (MQ=255)         aGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTACGTCtttt                                                        <  1:352397/80‑1 (MQ=255)                       tACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATg                                          <  2:352398/80‑1 (MQ=255)                         ctctctGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGc                                        <  2:352399/80‑1 (MQ=255)                              tGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGt                                     <  2:352400/78‑1 (MQ=255)                                       agcagTTGTGGGATAATATCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTg                         >  2:352432/1‑81 (MQ=255)                                             tgtgGGATAATATCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGc                    >  1:352445/1‑80 (MQ=255)                                                  gATAATATCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGCAAAGCt              <  1:352401/81‑1 (MQ=255)                                                        atCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGCAAAGCTGCCaa         >  2:352446/1‑80 (MQ=255)                                                         tCGGCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGCAAAGCTGCCAAc        <  2:352402/80‑1 (MQ=255)                                                            gCAGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGCAAAGCTGCCAACAGGc    >  2:352442/1‑81 (MQ=255)                                                              aGGATTCTGGGGAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGCAAAGCTGCCAACAGGCTg  >  2:352434/1‑81 (MQ=255)                                                                         |                                                                      ACCGTATCAGTTAAACAGCCTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGGCAGGATTCTGGGAAAGTTTACGTCTTTTTACTGCCCGGGATGGCGGTTGACATAAGCTGCAGGCAAAGCTGCCAACAGGCTG  >  NC_000913/1977256‑1977397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑