Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,993,117	G→A	I530I (ATC→ATT)	uvrC ←	excision nuclease subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,993,117	0	G	A	100.0%	80.2 / NA	23	I530I (ATC→ATT)	uvrC	excision nuclease subunit C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

GTATTTTTGACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCC  >  NC_000913/1993043‑1993194                                                                           |                                                                              gTATTTTTGACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACa                                                                         <  1:354801/81‑1 (MQ=255)   aTTTTTGACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATg                                                                       >  2:354832/1‑81 (MQ=255)           ccTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCgg                                                               <  1:354803/81‑1 (MQ=255)           ccTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCgg                                                               <  2:354802/81‑1 (MQ=255)                      tttttACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTggc                                                    >  2:354824/1‑81 (MQ=255)                            cGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCaaa                                              <  1:354804/81‑1 (MQ=255)                                       aaTCGCGTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCt                                    >  2:354844/1‑80 (MQ=255)                                             gTGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTc                             <  1:354805/81‑1 (MQ=255)                                              tGATCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTcc                            <  1:354806/81‑1 (MQ=255)                                                aTCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCgt                          >  1:354841/1‑80 (MQ=255)                                                aTCATGTGATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCgg                          >  1:354851/1‑81 (MQ=255)                                                     gtgATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCa                      >  2:354835/1‑80 (MQ=255)                                                      tgATTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCaa                     <  2:354807/80‑1 (MQ=255)                                                        aTTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg                   >  1:354850/1‑80 (MQ=255)                                                        aTTCATCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg                   >  2:354842/1‑80 (MQ=255)                                                            aTCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAg              <  1:354808/81‑1 (MQ=255)                                                             tCGCGAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGc             >  2:354848/1‑81 (MQ=255)                                                                 gAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGttt         >  2:354833/1‑81 (MQ=255)                                                                 gAATATGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGttt         >  2:354847/1‑81 (MQ=255)                                                                   atatGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc        >  1:354843/1‑80 (MQ=255)                                                                    tatGCTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc        >  2:354840/1‑79 (MQ=255)                                                                        cTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtcc  <  2:354810/81‑1 (MQ=255)                                                                        cTGAATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc   <  1:354809/80‑1 (MQ=255)                                                                           |                                                                              GTATTTTTGACCTTCGCCCGTTTTTTACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCC  >  NC_000913/1993043‑1993194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑