Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,681	T→C	Y9Y (TAT→TAC)	yecU →	protein YecU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,681	0	T	C	100.0%	40.6 / NA	13	Y9Y (TAT→TAC)	yecU	protein YecU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/5);  total (8/5)

CGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATG  >  NC_000913/1995604‑1995753                                                                              |                                                                         cGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTacat                                                                        >  1:355248/1‑80 (MQ=255) cGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACAtt                                                                       <  2:355237/81‑1 (MQ=255)                              aCAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCAc                                          >  1:355238/1‑81 (MQ=255)                                      ggCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCaaa                                   >  2:355251/1‑80 (MQ=255)                                         aaaaaaCATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCaaaaaa                                >  1:355255/1‑80 (MQ=255)                                           aaaaCATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAaacaa                             >  1:355260/1‑81 (MQ=255)                                             aaCATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAaacaac                            >  2:355261/1‑80 (MQ=255)                                                   cATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGc                      <  1:355240/80‑1 (MQ=255)                                                   cATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGc                      <  2:355239/80‑1 (MQ=255)                                                    aTGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCt                     <  1:355241/80‑1 (MQ=255)                                                                    ttGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTa    >  2:355282/1‑81 (MQ=255)                                                                     tGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt   >  1:355262/1‑81 (MQ=255)                                                                     tGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt   >  2:355252/1‑81 (MQ=255)                                                                       gTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATg  <  1:355242/80‑1 (MQ=255)                                                                              |                                                                         CGCTGAATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATG  >  NC_000913/1995604‑1995753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑