Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,748	C→G	L32V (CTT→GTT)	yecU →	protein YecU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,748	0	C	G	100.0%	63.0 / NA	19	L32V (CTT→GTT)	yecU	protein YecU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (14/5);  total (14/5)

TTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAG  >  NC_000913/1995671‑1995822                                                                              |                                                                           ttGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTa                                                                         >  2:355282/1‑81 (MQ=255)  tGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt                                                                        >  1:355262/1‑81 (MQ=255)  tGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt                                                                        >  2:355252/1‑81 (MQ=255)    gTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATg                                                                       <  1:355242/80‑1 (MQ=255)            atTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTcc                                                              >  2:355253/1‑81 (MQ=255)               aaCGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTccgcc                                                           >  1:355258/1‑81 (MQ=255)                aCGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTccgcc                                                           <  2:355243/80‑1 (MQ=255)                   tATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCt                                                       >  1:355264/1‑81 (MQ=255)                       ttATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTaa                                                    >  1:355254/1‑80 (MQ=255)                            gCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACAtttt                                              >  1:355256/1‑81 (MQ=255)                                 gCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCAcc                                         >  2:355270/1‑81 (MQ=255)                                    gATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGc                                       >  1:355269/1‑80 (MQ=255)                                     atatCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCa                                      >  1:355268/1‑80 (MQ=255)                                                aaaaaaCAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGc                          <  2:355244/81‑1 (MQ=255)                                                   aaaCAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCaa                        >  2:355263/1‑80 (MQ=255)                                                            cTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAgg              <  2:355245/81‑1 (MQ=255)                                                                  tttttAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAaaat         <  1:355246/80‑1 (MQ=255)                                                                       aGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAaaataaat     >  2:355272/1‑79 (MQ=255)                                                                        gCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAg  >  1:355267/1‑81 (MQ=255)                                                                              |                                                                           TTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAAATGAG  >  NC_000913/1995671‑1995822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑