Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,608	G→A	N75N (AAC→AAT)	sdiA ←	DNA‑binding transcriptional dual regulator SdiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,608	0	G	A	100.0%	65.0 / NA	19	N75N (AAC→AAT)	sdiA	DNA‑binding transcriptional dual regulator SdiA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/8);  total (11/8)

GTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATG  >  NC_000913/1996539‑1996681                                                                      |                                                                          gTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCt                                                                 >  2:355371/1‑80 (MQ=255) gTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTg                                                                <  2:355345/81‑1 (MQ=255)           cacaTTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAAtaact                                                       <  1:355346/80‑1 (MQ=255)             caTTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAAtaactaac                                                    <  1:355347/81‑1 (MQ=255)              aTTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAAcc                                                   <  1:355348/81‑1 (MQ=255)                    tGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGcc                                             >  2:355386/1‑81 (MQ=255)                     ggCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCt                                            >  1:355385/1‑81 (MQ=255)                            aCTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAggg                                       <  1:355349/79‑1 (MQ=255)                               aaaGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAAtt                                  >  2:355381/1‑81 (MQ=255)                                       cAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTaa                           >  1:355383/1‑80 (MQ=255)                                       cAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTaa                           <  2:355350/80‑1 (MQ=255)                                       cAGGGTTCAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTaa                           >  2:355377/1‑80 (MQ=255)                                              cAGCACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCAc                   >  1:355376/1‑81 (MQ=255)                                                 cACCGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACtt                 >  2:355390/1‑80 (MQ=255)                                                   ccGGATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAg              >  1:355384/1‑81 (MQ=255)                                                     ggATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGt            >  1:355382/1‑81 (MQ=255)                                                      gATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTc           <  2:355351/81‑1 (MQ=255)                                                      gATCAATTGCGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTc           >  2:355378/1‑81 (MQ=255)                                                               cGAGAAAATTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATg  <  2:355352/81‑1 (MQ=255)                                                                      |                                                                          GTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATG  >  NC_000913/1996539‑1996681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑