Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,007,153	C→T	T333I (ACC→ATC)	amyA →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,007,153	0	C	T	100.0%	104.9 / NA	30	T333I (ACC→ATC)	amyA	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/12);  total (18/12)

ATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTG  >  NC_000913/2007077‑2007226                                                                             |                                                                          aTGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCaa                                                                       >  2:357029/1‑81 (MQ=255)    aCGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAAcc                                                                     <  1:357007/80‑1 (MQ=255)           ttttCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAg                                                             >  2:357043/1‑81 (MQ=255)            tttCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGc                                                            >  1:357037/1‑81 (MQ=255)               cACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCt                                                         >  1:357041/1‑81 (MQ=255)                aCGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTc                                                        <  1:357008/81‑1 (MQ=255)                aCGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTc                                                        <  1:357009/81‑1 (MQ=255)                  gggTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCg                                                       >  1:357045/1‑80 (MQ=255)                    gTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAg                                                    >  1:357036/1‑81 (MQ=255)                    gTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAg                                                    <  1:357010/81‑1 (MQ=255)                     tACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAgc                                                   >  1:357055/1‑81 (MQ=255)                         ttAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCg                                               <  2:357011/81‑1 (MQ=255)                            gTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTc                                            <  2:357013/81‑1 (MQ=255)                            gTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTc                                            <  2:357012/81‑1 (MQ=255)                                   ccGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGt                                     >  1:357061/1‑81 (MQ=255)                                     gATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTgg                                   >  1:357040/1‑81 (MQ=255)                                     gATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTgg                                   >  1:357048/1‑81 (MQ=255)                                       tCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGtt                                 >  1:357038/1‑81 (MQ=255)                                            tCCACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAAc                            >  1:357046/1‑81 (MQ=255)                                             ccACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAAcc                           >  1:357053/1‑81 (MQ=255)                                              cACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGTCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCg                          >  2:357047/1‑81 (MQ=255)                                              cACGCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTAGCAAGCCCTCAAAGCGCCGGTCGAACCGTGATTTAAACCg                          <  1:357014/81‑1 (MQ=255)                                                 gCCGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTg                       <  2:357015/81‑1 (MQ=255)                                                  ccGTGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTgg                      <  1:357016/81‑1 (MQ=255)                                                     tGACGCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCg                    >  2:357057/1‑80 (MQ=255)                                                         gCTCGTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGc               <  2:357017/81‑1 (MQ=255)                                                             gTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCtt            >  1:357054/1‑80 (MQ=255)                                                             gTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCtt            >  1:357052/1‑80 (MQ=255)                                                             gTTGCCAATCACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTa           <  2:357018/81‑1 (MQ=255)                                                                      cACGACATCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTttgttg  >  1:357056/1‑81 (MQ=255)                                                                             |                                                                          ATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTG  >  NC_000913/2007077‑2007226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑