Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,811	T→G	E90A (GAG→GCG)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,811	0	T	G	100.0%	115.7 / NA	34	E90A (GAG→GCG)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/15);  total (19/15)

GGGGGCGATTTGCGCGCTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCTCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTC  >  NC_000913/2032735‑2032888                                                                             |                                                                              gggggCGATTTGCGCGCTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGaa                                                                           <  2:361469/81‑1 (MQ=255)     gCGATTTGCGCGCTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAActct                                                                       <  2:361470/81‑1 (MQ=255)         tttGCGCGCTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCAc                                                                   <  2:361471/81‑1 (MQ=255)            gcgcgcTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACgg                                                                 <  1:361472/80‑1 (MQ=255)            gcgcgcTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACgg                                                                 <  2:361473/80‑1 (MQ=255)               cgcTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAg                                                               >  2:361503/1‑79 (MQ=255)                gcTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAg                                                            <  1:361474/81‑1 (MQ=255)                       cTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGAcgc                                                     <  1:361475/81‑1 (MQ=255)                               ggCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCtcttc                                             >  2:361498/1‑81 (MQ=255)                               ggCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCtcttc                                             >  2:361504/1‑81 (MQ=255)                               ggCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCtcttc                                             >  1:361496/1‑81 (MQ=255)                                    tGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTgccg                                        <  2:361477/81‑1 (MQ=255)                                    tGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTgccg                                        <  2:361476/81‑1 (MQ=255)                                          gACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGa                                  >  2:361520/1‑81 (MQ=255)                                           aCAAACGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGAt                                 >  1:361512/1‑81 (MQ=255)                                              aaCGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTga                              <  2:361478/81‑1 (MQ=255)                                               aCGGCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTg                               >  1:361497/1‑79 (MQ=255)                                                  gCCTCGATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTgaagaa                          >  1:361518/1‑81 (MQ=255)                                                       gATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTg                     >  1:361523/1‑81 (MQ=255)                                                       gATTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTg                     <  2:361479/81‑1 (MQ=255)                                                        aTTTTCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGc                    >  1:361524/1‑81 (MQ=255)                                                         ttttCTCCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGcc                   >  2:361511/1‑81 (MQ=255)                                                            tctcCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCttt                >  2:361517/1‑81 (MQ=255)                                                             ctcCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCttt                <  1:361480/80‑1 (MQ=255)                                                             ctcCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTg               >  1:361529/1‑81 (MQ=255)                                                              tcCGCCGGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGc              >  2:361506/1‑81 (MQ=255)                                                               ccgccgGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCg             >  2:361521/1‑81 (MQ=255)                                                               ccgccgGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCg             >  1:361510/1‑81 (MQ=255)                                                                cgccgGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGc              <  2:361481/79‑1 (MQ=255)                                                                cgccgGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCg             >  1:361516/1‑80 (MQ=255)                                                                   cgGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGgatg         <  1:361482/81‑1 (MQ=255)                                                                   cgGAAATTGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGgatg         <  1:361483/81‑1 (MQ=255)                                                                         ttGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGAttttt   >  1:361515/1‑81 (MQ=255)                                                                          tGCGCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTc  >  2:361519/1‑81 (MQ=255)                                                                             |                                                                              GGGGGCGATTTGCGCGCTGAAACTGTGAGCGGCAATGGCATGACAAACGGCCTCGATTTTCTCCGCCGGAAATTGCTCGAACTCTTCACGGAGCAGGCGACGCGTCTCTTCTGCCGCCAGGATTGAAGAACGCTGCCTTTGCGGATGATTTTTC  >  NC_000913/2032735‑2032888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑