Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,037,115	T→C	K346K (AAA→AAG)	hprS ←	sensory histidine kinase HprS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,037,115	0	T	C	100.0%	40.4 / NA	13	K346K (AAA→AAG)	hprS	sensory histidine kinase HprS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/5);  total (8/5)

GATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTC  >  NC_000913/2037050‑2037183                                                                  |                                                                     gATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGAtt                                                       <  1:362054/81‑1 (MQ=255)           gCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTg                                              <  2:362055/80‑1 (MQ=255)             gAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCAc                                           >  2:362071/1‑81 (MQ=255)                 atCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGa                                       >  2:362065/1‑81 (MQ=255)                   cTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGAcc                                     >  2:362066/1‑81 (MQ=255)                   cTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGAcc                                     >  2:362069/1‑81 (MQ=255)                      aTGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGACCtt                                   >  2:362078/1‑80 (MQ=255)                         gCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTaaa                                <  1:362056/80‑1 (MQ=255)                         gCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAg                               >  1:362064/1‑81 (MQ=255)                            ttAACAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCa                             >  2:362072/1‑80 (MQ=255)                               aCAATAAGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAAt                          <  1:362057/80‑1 (MQ=255)                                     aGATTCGATAACATTCGTTGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCttt                    <  1:362058/80‑1 (MQ=255)                                                       tGTAGTAAAATCTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTc  >  2:362091/1‑80 (MQ=255)                                                                  |                                                                     GATTTTTCTGGCGAATATCTAATGGCATTAACAATAAGATTCGATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTC  >  NC_000913/2037050‑2037183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑