Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,076,083	T→C	N435N (AAT→AAC)	yeeR →	CP4‑44 prophage; inner membrane protein YeeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,076,083	0	T	C	100.0%	81.5 / NA	24	N435N (AAT→AAC)	yeeR	CP4‑44 prophage; inner membrane protein YeeR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/14);  total (10/14)

TTGCCGCTGAACGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAATAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCAACTGCAGCCCGTA  >  NC_000913/2076013‑2076159                                                                       |                                                                             ttGCCGCTGAACGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGa                                                                     >  1:368160/1‑80 (MQ=255)  tGCCGCTGAACGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGaa                                                                    <  1:368121/80‑1 (MQ=255)   gCCGCTGAACGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAAt                                                                  >  1:368174/1‑81 (MQ=255)        tGAACGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAg                                                              >  2:368177/1‑80 (MQ=255)         gAACGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAg                                                              >  1:368170/1‑79 (MQ=255)            cGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGcagac                                                         <  1:368123/81‑1 (MQ=255)            cGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGcagac                                                         <  2:368122/81‑1 (MQ=255)              gggAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGcagacag                                                       <  2:368124/81‑1 (MQ=255)              gggAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGcaga                                                          >  1:368173/1‑78 (MQ=255)                 aaaCCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACt                                                    <  1:368125/81‑1 (MQ=255)                    ccATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGt                                                 >  2:368168/1‑81 (MQ=255)                         ttGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGc                                             <  2:368126/80‑1 (MQ=255)                            tCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGa                                          <  1:368127/80‑1 (MQ=255)                              gACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACa                                       <  2:368128/81‑1 (MQ=255)                                 agagTCCGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGa                                     >  2:368176/1‑80 (MQ=255)                                      ccGTCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATc                                 >  1:368172/1‑79 (MQ=255)                                         tCTGAAAAACGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGAc                            <  2:368129/81‑1 (MQ=255)                                                 aCGCACGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATAtt                    <  2:368130/81‑1 (MQ=255)                                                      cGGATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCa               <  2:368131/81‑1 (MQ=255)                                                       ggATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCaa              >  1:368171/1‑81 (MQ=255)                                                       ggATCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCaa              <  2:368132/81‑1 (MQ=255)                                                         aTCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCAACt            >  1:368179/1‑81 (MQ=255)                                                         aTCCTCGGTGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCAACt            <  2:368133/81‑1 (MQ=255)                                                                 tGATAACAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCAACTGCAGCCCg    <  2:368134/81‑1 (MQ=255)                                                                   ataacaATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCAACTGCAGCCCGTa  <  2:368135/81‑1 (MQ=255)                                                                       |                                                                             TTGCCGCTGAACGGGGAAACCATATTGTCGACAGAGTCCGTCTGAAAAACGCACGGATCCTCGGTGATAATAATGCCAGAAATGGAGCAGACAGACTGGTCAGCGGAACAGAAATCCAGACGAAATATTGTTCAACTGCAGCCCGTA  >  NC_000913/2076013‑2076159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑