Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,809	C→T	R174K (AGA→AAA)	glf ←	UDP‑galactopyranose mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,809	0	C	T	100.0%	65.6 / NA	19	R174K (AGA→AAA)	glf	UDP‑galactopyranose mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/7);  total (12/7)

TTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAC  >  NC_000913/2107732‑2107881                                                                              |                                                                         tttCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAAtttc                                                                        <  1:373093/80‑1 (MQ=255) tttCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCa                                                                       >  2:373118/1‑81 (MQ=255)              aGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGc                                                            <  2:373094/79‑1 (MQ=255)               gtgtAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTta                                                         <  2:373095/81‑1 (MQ=255)                 gtAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTtaa                                                        >  2:373114/1‑80 (MQ=255)                    gCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTtaataa                                                     >  2:373115/1‑80 (MQ=255)                      cTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTtaataata                                                   >  2:373109/1‑80 (MQ=255)                             cGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAgg                                           <  2:373096/81‑1 (MQ=255)                                aaTACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCaa                                        >  1:373113/1‑81 (MQ=255)                                aaTACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCaa                                        >  1:373123/1‑81 (MQ=255)                                    ccTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTc                                     <  1:373098/80‑1 (MQ=255)                                    ccTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCt                                    <  2:373097/81‑1 (MQ=255)                                        gATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTg                                >  2:373116/1‑81 (MQ=255)                                         aTAGCGATCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGc                               <  2:373099/81‑1 (MQ=255)                                                tCGGAAAAATAATTGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCActtctt                        >  1:373121/1‑81 (MQ=255)                                                             tGTTATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTtct           >  1:373124/1‑81 (MQ=255)                                                                tATCAAACGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCg        >  2:373120/1‑81 (MQ=255)                                                                    aaaCGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGtat     >  1:373119/1‑80 (MQ=255)                                                                     aaCGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATaa   <  1:373100/81‑1 (MQ=255)                                                                      aCGTAAATTTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAc  >  1:373117/1‑81 (MQ=255)                                                                              |                                                                         TTTCAATAAGCTTAGTGTAGCCTCCCACCGGAATACCTTGATAGCGATCGGAAAAATAATTGTTATCAAACGTAAATCTCACTGGGATTCGCTTAATAATAAATGCAGGCAATTCTTTTGCACTTCTTCCCCACTGCTTCTCCGTATAAC  >  NC_000913/2107732‑2107881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑