Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,482	T→C	K282K (AAA→AAG)	galF ←	UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,482	0	T	C	100.0%	79.0 / NA	24	K282K (AAA→AAG)	galF	UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/10);  total (14/10)

AACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACT  >  NC_000913/2113408‑2113550                                                                           |                                                                     aaCCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAg                                                                 <  1:373717/80‑1 (MQ=255)   ccGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCACCCCTTCCTTCAGGtt                                                              >  1:373736/1‑81 (MQ=255)    cGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTg                                                             >  2:373748/1‑81 (MQ=255)     gTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGc                                                            <  2:373718/81‑1 (MQ=255)        aCATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCg                                                           >  2:373757/1‑79 (MQ=255)                tCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATAc                                                 <  1:373719/81‑1 (MQ=255)                     ttatCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTcac                                            <  2:373720/78‑1 (MQ=255)                         cATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAAc                                        >  2:373752/1‑81 (MQ=255)                            tattCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGcc                                     >  1:373758/1‑81 (MQ=255)                              ttCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTg                                   >  2:373778/1‑81 (MQ=255)                                cGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCa                                 >  2:373742/1‑81 (MQ=255)                                 gCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCa                                 >  2:373749/1‑80 (MQ=255)                                 gCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCa                                 >  1:373755/1‑80 (MQ=255)                                   ttAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCata                               >  2:373743/1‑80 (MQ=255)                                    tAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCata                               >  1:373747/1‑79 (MQ=255)                                       cAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGcc                          <  1:373721/81‑1 (MQ=255)                                           ttCTCAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCAAAAACGCCTGCATATAGCCCAtt                      >  2:373774/1‑81 (MQ=255)                                                 aTACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTg                <  2:373722/81‑1 (MQ=255)                                                  tACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGc               <  1:373723/81‑1 (MQ=255)                                                    ccTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCg             >  1:373750/1‑81 (MQ=255)                                                        tACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGt         >  2:373754/1‑81 (MQ=255)                                                         aCGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGt         <  1:373724/80‑1 (MQ=255)                                                           ggAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGt      <  1:373725/81‑1 (MQ=255)                                                               cTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACt  <  2:373726/81‑1 (MQ=255)                                                                           |                                                                     AACCGTTACATCCGGTCAGATTTTCATTATTCGCTTAACAGCTTCTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACT  >  NC_000913/2113408‑2113550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑