Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,137,560	T→C	intergenic (‑317/‑342)	wza ← / → yegH	outer membrane polysaccharide export protein Wza/inner membrane protein YegH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,137,560	0	T	C	100.0%	32.4 / NA	11	intergenic (‑317/‑342)	wza/yegH	outer membrane polysaccharide export protein Wza/inner membrane protein YegH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

TCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATT  >  NC_000913/2137484‑2137629                                                                             |                                                                      tctcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATCGaa                                                                    >  2:378133/1‑80 (MQ=255)            aGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATCGAATATTGGCTTTc                                                         >  2:378143/1‑80 (MQ=255)                            gggTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATCGAATATTGGCTTTCCAATAATGCaggagg                                          >  2:378140/1‑79 (MQ=255)                                      ggAAATTAACTTCCGAATATAAGGTGACATTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGc                              <  2:378117/81‑1 (MQ=255)                                        aaaTTAACTTCCGAATATAAGGTGACATTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa                            <  2:378118/81‑1 (MQ=255)                                                ttCCGAATATAAGGTGACATTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGaa                      >  1:378142/1‑79 (MQ=255)                                                           aGGTGACATTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGAt         <  2:378119/81‑1 (MQ=255)                                                             gTGACATTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATaa       <  1:378120/81‑1 (MQ=255)                                                                aCATTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACaa    >  1:378146/1‑81 (MQ=255)                                                                 cATTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAAt   >  2:378138/1‑81 (MQ=255)                                                                  aTTATGGTAATCGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAAtt  <  1:378121/81‑1 (MQ=255)                                                                             |                                                                      TCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATT  >  NC_000913/2137484‑2137629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑