Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,118	G→A	P290S (CCG→TCG)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,118	0	G	A	100.0%	84.2 / NA	25	P290S (CCG→TCG)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/7);  total (18/7)

TTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACA  >  NC_000913/2150046‑2150186                                                                         |                                                                     ttGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCgg                                                              >  2:380508/1‑81 (MQ=255)   gCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGtt                                                            >  2:380506/1‑81 (MQ=255)    cTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGtt                                                            <  2:380462/80‑1 (MQ=255)    cTGAAACGATACATTTCTTTAACTGTAGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTg                                                           <  1:380463/81‑1 (MQ=255)     tGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTgc                                                          >  2:380494/1‑81 (MQ=255)       aaaCGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTgcca                                                        >  2:380534/1‑81 (MQ=255)       aaaCGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTgc                                                          <  2:380464/79‑1 (MQ=255)        aaCGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTgccac                                                       >  2:380485/1‑81 (MQ=255)           gATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTgccacgc                                                     >  1:380490/1‑80 (MQ=255)               cATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACtt                                                <  2:380465/81‑1 (MQ=255)                   tCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCg                                            >  2:380487/1‑81 (MQ=255)                    cTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGt                                           >  2:380489/1‑81 (MQ=255)                        aaCTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAAt                                       >  1:380495/1‑81 (MQ=255)                         aCTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATg                                      >  1:380492/1‑81 (MQ=255)                            gTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCct                                   >  1:380501/1‑81 (MQ=255)                                     gagagTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAAc                           <  2:380466/80‑1 (MQ=255)                                       gagTCCAGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATg                        >  2:380504/1‑81 (MQ=255)                                             aGTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTc                   >  2:380497/1‑80 (MQ=255)                                              gTGCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCaa                 >  1:380500/1‑81 (MQ=255)                                                gCCGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACa               >  2:380496/1‑81 (MQ=255)                                                  cGCTACCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACAt              >  1:380507/1‑80 (MQ=255)                                                      aCCCACGCTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTc         >  1:380524/1‑81 (MQ=255)                                                       cccACGCTTTAGCCGTAGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTc         <  2:380467/80‑1 (MQ=255)                                                             cTTTAGCCGTCGAACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACa  >  1:380505/1‑81 (MQ=255)                                                             cTTTAGCAGTCGAACGCCCGGTTGCCAAGCCACTTACCGTGAATGCCTGATGAAACATGGCTTAAACATCACTCGGTAACa  <  2:1015264/81‑1 (MQ=255)                                                                         |                                                                     TTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACA  >  NC_000913/2150046‑2150186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑