Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,158,165	T→C	I968T (ATC→ACC)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,158,165	0	T	C	100.0%	95.2 / NA	28	I968T (ATC→ACC)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/10);  total (18/10)

CTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCG  >  NC_000913/2158089‑2158240                                                                             |                                                                            ctggctgCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCt                                                                          >  1:381914/1‑80 (MQ=255)      tgctgAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGAc                                                                    >  1:381922/1‑81 (MQ=255)        ctgAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGAc                                                                    <  2:381889/79‑1 (MQ=255)            gcgcGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCActct                                                              <  1:381890/81‑1 (MQ=255)             cgcgAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTg                                                             <  1:381891/81‑1 (MQ=255)                    aaGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGctc                                                      >  1:381926/1‑81 (MQ=255)                    aaGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGctc                                                      >  2:381933/1‑81 (MQ=255)                    aaGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGctc                                                      >  2:381935/1‑81 (MQ=255)                     aGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGctct                                                     <  1:381892/81‑1 (MQ=255)                     aGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGcact                                                     >  1:381924/1‑81 (MQ=255)                                  cgcgAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTg                                        >  2:381942/1‑81 (MQ=255)                                  cgcgAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTg                                        >  2:381928/1‑81 (MQ=255)                                     gAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCg                                     <  1:381893/81‑1 (MQ=255)                                     gAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCg                                     >  2:381927/1‑81 (MQ=255)                                        gCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCctct                                   >  2:381923/1‑80 (MQ=255)                                           aTCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATg                               >  1:381921/1‑81 (MQ=255)                                           aTCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATg                               <  1:381894/81‑1 (MQ=255)                                               aCCAGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGtt                             >  2:381938/1‑79 (MQ=255)                                                  aGGCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTa                        >  2:381943/1‑81 (MQ=255)                                                   ggCTTGTCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTAc                       >  1:381936/1‑81 (MQ=255)                                                         tCTGTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCgggg                  <  2:381895/80‑1 (MQ=255)                                                            gTTGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCgg              >  2:381945/1‑81 (MQ=255)                                                             ttGCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGgc             <  1:381896/81‑1 (MQ=255)                                                               gCGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGgcgc           >  1:381940/1‑81 (MQ=255)                                                                cGTTTTCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTTGGcgcg          <  2:381897/81‑1 (MQ=255)                                                                    ttCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGaa       <  1:381898/80‑1 (MQ=255)                                                                    ttCGTCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAAc      >  1:381944/1‑81 (MQ=255)                                                                        tCCGACCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGcg  >  1:381954/1‑81 (MQ=255)                                                                             |                                                                            CTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCG  >  NC_000913/2158089‑2158240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑