Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,161,778	C→T	R105R (CGC→CGT)	mdtD →	putative multidrug efflux pump MdtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,161,778	0	C	T	100.0%	104.1 / NA	30	R105R (CGC→CGT)	mdtD	putative multidrug efflux pump MdtD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

TACCGCCATCGTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGCGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGAAAATCGTACCGCGCGAGC  >  NC_000913/2161700‑2161854                                                                               |                                                                             tACCGCCATCGTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCAcgtgc                                                                            >  1:382713/1‑81 (MQ=255)  aCCGCCATCGTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCAcgtgcg                                                                           >  1:382706/1‑81 (MQ=255)  aCCGCCATCGTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCAcgtgc                                                                            <  2:382673/80‑1 (MQ=255)  aCCGCCATCGTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCAcgtgc                                                                            <  2:382672/80‑1 (MQ=255)     gCCATCGTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTa                                                                        >  2:382708/1‑81 (MQ=255)           gTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGc                                                                  <  1:382675/81‑1 (MQ=255)           gTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGc                                                                  <  1:382674/81‑1 (MQ=255)           gTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGc                                                                  >  1:382712/1‑81 (MQ=255)           gTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCCGGCACGTGCGTTACAGGGc                                                                  >  2:382707/1‑81 (MQ=255)                gTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTgg                                                             >  2:382717/1‑81 (MQ=255)                 tttACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTggc                                                            <  1:382676/81‑1 (MQ=255)                    aCTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTggcggc                                                         >  2:382716/1‑81 (MQ=255)                     ctctCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTggcggc                                                         <  1:382677/80‑1 (MQ=255)                            ttCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCgatgat                                                 <  1:382678/81‑1 (MQ=255)                              cACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATgg                                               >  2:382720/1‑81 (MQ=255)                               aCTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGt                                              >  2:382726/1‑81 (MQ=255)                               aCTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGt                                              >  1:382705/1‑81 (MQ=255)                                    tttGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCgg                                         >  2:382710/1‑81 (MQ=255)                                       gcgcgcTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTc                                       <  1:382679/80‑1 (MQ=255)                                       gcgcgcTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTc                                       <  1:382680/80‑1 (MQ=255)                                         gcgcTTTCCGGCTCGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCgg                                     >  2:382714/1‑80 (MQ=255)                                          cgcTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCa                                   <  1:382681/81‑1 (MQ=255)                                             tttCCGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGAt                                >  1:382735/1‑81 (MQ=255)                                                ccGGCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTg                              <  2:382682/80‑1 (MQ=255)                                                  ggCACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACg                           >  1:382725/1‑81 (MQ=255)                                                    cACGCTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGt                         <  1:382683/81‑1 (MQ=255)                                                        cTGAACGAACTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGtgatg                     <  2:382684/81‑1 (MQ=255)                                                           aacgaacTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGtgatga                    <  1:382686/79‑1 (MQ=255)                                                           aacgaacTGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGaa                   <  1:382685/80‑1 (MQ=255)                                                                  tGTTGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGAAAATCGTAc           >  2:382715/1‑81 (MQ=255)                                                                     tGCTGGCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGAAAATCGTACcgc        >  2:382723/1‑81 (MQ=255)                                                                         ggCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGAAAATCGTACcgcgc      >  1:382711/1‑79 (MQ=255)                                                                          gCACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGAAAATCGTACcgcgcg     <  1:382687/79‑1 (MQ=255)                                                                           cACGTGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGAAAATCGTACCGCGCGAGc  <  2:382688/81‑1 (MQ=255)                                                                               |                                                                             TACCGCCATCGTGCTGTTTACTCTCGGTTCACTGTTTTGCGCGCTTTCCGGCACGCTGAACGAACTGTTGCTGGCACGCGCGTTACAGGGCGTTGGCGGCGCGATGATGGTGCCGGTCGGCAGATTGACGGTGATGAAAATCGTACCGCGCGAGC  >  NC_000913/2161700‑2161854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑