Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,162,642	G→A	L393L (CTG→CTA)	mdtD →	putative multidrug efflux pump MdtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,162,642	0	G	A	100.0%	91.6 / NA	27	L393L (CTG→CTA)	mdtD	putative multidrug efflux pump MdtD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/20);  total (7/20)

CTCGACGCGTTTCTCCTCCATGAACACCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTGCTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGTTGCTGGGACTTTTTGGT  >  NC_000913/2162564‑2162714                                                                               |                                                                         cTCGACGCGTTTCTCCTCCATGAACACCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCctact                                                                        <  1:382843/81‑1 (MQ=255)       gcgTTTCTCCTCCATGAACACCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCgat                                                                  <  1:382844/81‑1 (MQ=255)         gTTTCTCCTCCATGAACACCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCgatga                                                                >  2:382881/1‑81 (MQ=255)                       aaCACCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTc                                                   >  1:382888/1‑80 (MQ=255)                       aaCACCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCg                                                  <  2:382845/81‑1 (MQ=255)                          acCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATg                                               <  1:382846/81‑1 (MQ=255)                           cccTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGa                                              <  2:382847/81‑1 (MQ=255)                             cTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGt                                            <  1:382848/81‑1 (MQ=255)                                aCGCTGAAAGATCTCCCGGTCAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATc                                         <  2:382849/81‑1 (MQ=255)                                aCGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATc                                         >  2:382882/1‑81 (MQ=255)                                  gCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCgg                                       <  2:382850/81‑1 (MQ=255)                                  gCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCgg                                       >  2:382891/1‑81 (MQ=255)                                      aaaGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCg                                     <  2:382852/79‑1 (MQ=255)                                      aaaGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTc                                   <  2:382851/81‑1 (MQ=255)                                         gATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACt                                <  1:382853/81‑1 (MQ=255)                                               ccGGACGATCTGGCGAGCAGCGGCAACGGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGcc                          <  1:382855/81‑1 (MQ=255)                                               ccGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTAt                              <  1:382854/77‑1 (MQ=255)                                               ccGGACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGcc                          <  1:382856/81‑1 (MQ=255)                                                 ggACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCgg                        >  2:382896/1‑81 (MQ=255)                                                  gACAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCggg                       <  2:382857/81‑1 (MQ=255)                                                    cAATCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCt                     <  2:382858/81‑1 (MQ=255)                                                      aTCTGGCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGt                   <  1:382859/81‑1 (MQ=255)                                                           gCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGTTGCTg              <  2:382861/81‑1 (MQ=255)                                                           gCGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGTTGCTg              <  2:382862/81‑1 (MQ=255)                                                           gCGAGCAGCGGCAACAACCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGtt                  <  2:382860/77‑1 (MQ=255)                                                            cGAGCAGCGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGTTGCTgg             <  2:382863/81‑1 (MQ=255)                                                                 agcGGCAACAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGTTGCTGGGACtt        >  2:382905/1‑81 (MQ=255)                                                                       aaCAGCCTACTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGTTGCTGGGACTTTTTGGt  >  1:382898/1‑81 (MQ=255)                                                                               |                                                                         CTCGACGCGTTTCTCCTCCATGAACACCCTGACGCTGAAAGATCTCCCGGACAATCTGGCGAGCAGCGGCAACAGCCTGCTGTCGATGATTATGCAATTGTCGATGAGTATCGGCGTCACTATCGCCGGGCTGTTGCTGGGACTTTTTGGT  >  NC_000913/2162564‑2162714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑