Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,022	A→C	N61K (AAT→AAG)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,022	0	A	C	100.0%	83.1 / NA	25	N61K (AAT→AAG)	gatY	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/8);  total (17/8)

TCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTT  >  NC_000913/2176949‑2177097                                                                          |                                                                            tCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTg                                                                      >  2:385358/1‑81 (MQ=255)   gtgtGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTa                                                                    >  2:385352/1‑81 (MQ=255)      tgATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTAcc                                                                  <  2:385321/80‑1 (MQ=255)        aTGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGc                                                               <  2:385322/81‑1 (MQ=255)          ggTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCAt                                                             >  2:385368/1‑81 (MQ=255)          ggTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCAt                                                             >  1:385365/1‑81 (MQ=255)             cGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAg                                                          >  2:385351/1‑81 (MQ=255)                    aaTTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAAtgt                                                   <  1:385323/81‑1 (MQ=255)                        gCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGc                                                 >  2:385355/1‑79 (MQ=255)                         ccAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAg                                              >  1:385350/1‑81 (MQ=255)                           aGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGc                                            <  1:385324/81‑1 (MQ=255)                                aTGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTcc                                       >  2:385370/1‑81 (MQ=255)                                 tGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCg                                      <  1:385325/81‑1 (MQ=255)                                        atTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCgatga                               >  2:385340/1‑81 (MQ=255)                                        atTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCgatga                               >  1:385361/1‑81 (MQ=255)                                        atTGCTTCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCgatga                               >  2:385367/1‑81 (MQ=255)                                             ttCGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGAcc                          >  1:385379/1‑81 (MQ=255)                                               cGCCATCGCGCTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGAcc                          <  1:385326/79‑1 (MQ=255)                                                        gcTGACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAg               >  2:385366/1‑81 (MQ=255)                                                           gACCAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGtt            >  1:385369/1‑81 (MQ=255)                                                              cAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTgg          >  1:385364/1‑80 (MQ=255)                                                              cAGCGCCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGc         <  2:385327/81‑1 (MQ=255)                                                                gcgcCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAg       <  1:385328/81‑1 (MQ=255)                                                                 cgcCAACAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCAGGCGATGATGACCGGCGCATGCAGGTTGGCAGc      >  2:385374/1‑81 (MQ=255)                                                                     aaCAGCTTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGtt  >  2:385375/1‑81 (MQ=255)                                                                          |                                                                            TCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTT  >  NC_000913/2176949‑2177097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑