Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,191,691	C→T	A178T (GCG→ACG)	yehD ←	putative fimbrial protein YehD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,191,691	0	C	T	100.0%	61.7 / NA	18	A178T (GCG→ACG)	yehD	putative fimbrial protein YehD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/12);  total (6/12)

AAGGCCGCCATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTT  >  NC_000913/2191621‑2191766                                                                       |                                                                            aaGGCCGCCATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCa                                                                     <  2:387717/79‑1 (MQ=255)         cATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAgaga                                                           <  1:387718/81‑1 (MQ=255)                    ggCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACAt                                                <  2:387719/81‑1 (MQ=255)                             tattaCCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGAtt                                       >  1:387741/1‑81 (MQ=255)                               ttaCCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGc                                     <  1:387720/81‑1 (MQ=255)                                  ccTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACt                                  <  1:387721/81‑1 (MQ=255)                                   cTGGAGAAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACt                                  >  1:387744/1‑80 (MQ=255)                                       agaAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGt                              >  1:387753/1‑80 (MQ=255)                                       agaAACTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGt                              >  2:387739/1‑80 (MQ=255)                                          aaCTTACAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATc                          >  2:387752/1‑81 (MQ=255)                                               aCAATTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTc                      <  2:387722/80‑1 (MQ=255)                                                  aTTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGtt                  <  2:387724/81‑1 (MQ=255)                                                  aTTTTATTTATTAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGtt                  <  1:387723/81‑1 (MQ=255)                                                        tttattAATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGt            <  2:387725/81‑1 (MQ=255)                                                           attaATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTAt          <  1:387726/80‑1 (MQ=255)                                                             taATCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTAtttt       >  1:387743/1‑81 (MQ=255)                                                               aTCCTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGc     <  2:387727/81‑1 (MQ=255)                                                                  cTGCGTCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCttt  <  1:387728/81‑1 (MQ=255)                                                                       |                                                                            AAGGCCGCCATGGGATAGCGGCCATGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTT  >  NC_000913/2191621‑2191766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑