Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,197,865	C→A	intergenic (+567/‑2414)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,197,865	0	C	A	100.0%	72.5 / NA	22	intergenic (+567/‑2414)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/10);  total (12/10)

AGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCC  >  NC_000913/2197787‑2197938                                                                               |                                                                          agcaATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTAtatc                                                                         <  2:388728/81‑1 (MQ=255)  gcaATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATca                                                                        <  2:388729/81‑1 (MQ=255)   caATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATcac                                                                       <  1:388731/81‑1 (MQ=255)   caATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATcac                                                                       <  1:388730/81‑1 (MQ=255)    aaTGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATcac                                                                       >  2:388752/1‑80 (MQ=255)     aTGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACaa                                                                     <  2:388732/81‑1 (MQ=255)       ggcggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAAc                                                                    >  1:388761/1‑80 (MQ=255)        gcggcACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAAcc                                                                   <  1:388733/80‑1 (MQ=255)                gACGGAACTTCTTGGGCAAAAAGTAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTgg                                                           >  2:388764/1‑80 (MQ=255)                  cGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAg                                                        <  1:388734/81‑1 (MQ=255)                             gggCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCAtt                                             >  1:388757/1‑81 (MQ=255)                              ggCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTc                                            >  2:388756/1‑81 (MQ=255)                                 aaaaaGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCt                                         >  1:388765/1‑81 (MQ=255)                                   aaaGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTgg                                       >  1:388774/1‑81 (MQ=255)                                       aagaGAACAGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGctct                                   <  2:388735/81‑1 (MQ=255)                                               aGTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACAccc                           >  1:388773/1‑81 (MQ=255)                                                gTTGGCGCATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCg                          >  2:388762/1‑81 (MQ=255)                                                    gcgcATTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAg                       <  1:388736/80‑1 (MQ=255)                                                        aTTATGCTAATGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGcc                  >  2:388768/1‑81 (MQ=255)                                                                  tGACAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAAt        >  1:388769/1‑81 (MQ=255)                                                                    aCAATGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCa      >  1:388778/1‑81 (MQ=255)                                                                       aTGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGc   <  2:388737/81‑1 (MQ=255)                                                                        tGCTTATATCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGcc  <  1:388738/81‑1 (MQ=255)                                                                               |                                                                          AGCAATGGCGGCACTGACGGAACTTCTTGGGCAAAAAGAAGAGAACAGTTGGCGCATTATGCTAATGACAATGCTTATCTCACAACCAGCACTGGCAGAACAGGTCATTCCCTGGCTCTCGACACCCGCAGTTGCCGTACTGAAATCATGCC  >  NC_000913/2197787‑2197938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑