Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,209,997	T→C	L300L (TTA→CTA)	yehQ →	SWIM zinc finger domains‑containing protein YehQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,209,997	0	T	C	100.0%	116.0 / NA	34	L300L (TTA→CTA)	yehQ	SWIM zinc finger domains‑containing protein YehQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/15);  total (19/15)

TTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGTT  >  NC_000913/2209920‑2210071                                                                              |                                                                           ttGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTa                                                                          >  2:390959/1‑80 (MQ=255)    aTGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAAtt                                                                      <  1:390913/81‑1 (MQ=255)    aTGCCTTCCACGCCCGCGCCAGCCACTATAAAGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAAtt                                                                      <  2:390912/81‑1 (MQ=255)            cACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGcaca                                                               <  2:390914/80‑1 (MQ=255)               gCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACaa                                                              >  2:390978/1‑78 (MQ=255)                 ccGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGAt                                                         <  1:390915/81‑1 (MQ=255)                   gcgcCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATgg                                                       >  2:390961/1‑81 (MQ=255)                    cgcCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGc                                                      <  2:390916/81‑1 (MQ=255)                    cgcCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGc                                                      <  2:390917/81‑1 (MQ=255)                          ccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGgcg                                                >  2:390970/1‑81 (MQ=255)                          ccaCTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGgcg                                                >  2:390950/1‑81 (MQ=255)                                taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGAc                                            <  1:390919/79‑1 (MQ=255)                                taATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAg                                          <  1:390918/81‑1 (MQ=255)                                   tGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTac                                       >  2:390957/1‑80 (MQ=255)                                   tGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTAt                                       <  1:390920/81‑1 (MQ=255)                                       ggAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGt                                   >  1:390963/1‑81 (MQ=255)                                          gaATGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTg                                  >  1:390968/1‑79 (MQ=255)                                             tGCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGtt                             <  1:390921/81‑1 (MQ=255)                                              gCTTACATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtc                            >  2:390979/1‑81 (MQ=255)                                                  aCATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTtcctcc                        >  1:390965/1‑81 (MQ=255)                                                   cATCAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTcctcct                       >  2:390958/1‑81 (MQ=255)                                                      cAGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCtgtg                    >  2:390946/1‑81 (MQ=255)                                                       aGCTTGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGc                   >  2:390974/1‑81 (MQ=255)                                                          ttGCGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGt                >  1:390966/1‑81 (MQ=255)                                                             cGGCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGgc             <  1:390922/81‑1 (MQ=255)                                                              ggCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGgcg            >  2:390995/1‑81 (MQ=255)                                                               gCATTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGgcgc           <  2:390923/81‑1 (MQ=255)                                                                 aTTAAACAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCAc         <  2:390924/81‑1 (MQ=255)                                                                     aaCAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTc     >  1:390955/1‑81 (MQ=255)                                                                     aaCAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTc     <  1:390925/81‑1 (MQ=255)                                                                     aaCAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTc     <  1:390926/81‑1 (MQ=255)                                                                     aaCAGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTc     >  2:390980/1‑81 (MQ=255)                                                                        aGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGtt  >  2:390973/1‑81 (MQ=255)                                                                        aGTCGACTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGtt  >  1:390967/1‑81 (MQ=255)                                                                              |                                                                           TTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGGCGCACGGTCGTT  >  NC_000913/2209920‑2210071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑