Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,221,701	T→C	M97V (ATG→GTG)	bglX ←	beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,221,701	0	T	C	100.0%	85.0 / NA	25	M97V (ATG→GTG)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/7);  total (18/7)

CGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGA  >  NC_000913/2221627‑2221775                                                                           |                                                                           cGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCt                                                                       >  1:393085/1‑80 (MQ=255)            tGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATgg                                                           <  2:393044/81‑1 (MQ=255)             gcgcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGgc                                                          >  1:393087/1‑81 (MQ=255)              cgcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGgcg                                                         <  1:393045/81‑1 (MQ=255)              cgcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGgcg                                                         >  1:393096/1‑81 (MQ=255)               gcTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGgcgc                                                        >  1:393086/1‑81 (MQ=255)                       tGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATcc                                                <  2:393046/81‑1 (MQ=255)                            caCGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACg                                           <  1:393047/81‑1 (MQ=255)                               gtcgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGt                                        >  1:393091/1‑81 (MQ=255)                                 cgtAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTa                                      >  1:393095/1‑81 (MQ=255)                                        aaagaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGtt                               >  2:393092/1‑81 (MQ=255)                                        aaagaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGtt                               >  2:393075/1‑81 (MQ=255)                                         aagaaaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGtt                               >  2:393088/1‑80 (MQ=255)                                             aaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGaaaa                          >  2:393094/1‑81 (MQ=255)                                             aaagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGaaaa                          >  1:393093/1‑81 (MQ=255)                                              aagaGGAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAAt                         <  2:393048/81‑1 (MQ=255)                                                  ggAATTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGcc                     >  1:393100/1‑81 (MQ=255)                                                    aaTTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGccc                    >  1:393111/1‑80 (MQ=255)                                                     aTTTTCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCa                  <  1:393049/81‑1 (MQ=255)                                                        ttCAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAAcc               >  1:393098/1‑81 (MQ=255)                                                          cAGGCGGCTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCt              <  1:393050/80‑1 (MQ=255)                                                             gcggcTTAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGAcc          >  1:393110/1‑81 (MQ=255)                                                                   tAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCttt    >  1:393101/1‑81 (MQ=255)                                                                   tAATTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCttt    >  2:393089/1‑81 (MQ=255)                                                                     aTTCCACCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGa  >  1:393106/1‑81 (MQ=255)                                                                           |                                                                           CGGGAACACCGTGCGCTGACCGTGCAGCACGTCGTAAGCAAAGAAAAGAGGAATTTTCAGGCGGCTTAATTCCATCACCTGATCCTGCATGGCGCGGATATCCTGACGGGTTACGGTGTTGAAAATCGCCCCAACCTGACCGTCTTTGA  >  NC_000913/2221627‑2221775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑