Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,824	T→C	intergenic (+58/‑16)	yeiH → / → nfo	conserved inner membrane protein YeiH/endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,824	0	T	C	100.0%	92.1 / NA	27	intergenic (+58/‑16)	yeiH/nfo	conserved inner membrane protein YeiH/endonuclease IV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

ATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTTAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTCGC  >  NC_000913/2250746‑2250899                                                                               |                                                                            aTTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGTGTTCaa                                                                           >  1:398412/1‑81 (MQ=255)  ttCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCaa                                                                           >  1:398407/1‑80 (MQ=255)   tCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCaa                                                                           >  1:398402/1‑79 (MQ=255)          gTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTcc                                                                  <  1:398363/81‑1 (MQ=255)             aTCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCg                                                               <  1:398364/81‑1 (MQ=255)                gCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCAt                                                            >  1:398400/1‑81 (MQ=255)                             cATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTgg                                                <  2:398365/80‑1 (MQ=255)                                   gCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAgc                                             <  2:398367/77‑1 (MQ=255)                                   gCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCa                                          <  1:398366/80‑1 (MQ=255)                                    cTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCAc                                         <  2:398368/80‑1 (MQ=255)                                    cTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACg                                        >  1:398403/1‑81 (MQ=255)                                     tCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGt                                       >  1:398406/1‑81 (MQ=255)                                      ccTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGt                                       >  2:398410/1‑80 (MQ=255)                                       cTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGtt                                      <  1:398371/80‑1 (MQ=255)                                       cTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGtt                                      <  1:398370/80‑1 (MQ=255)                                       cTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGt                                       >  2:398399/1‑79 (MQ=255)                                       cTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTa                                     <  1:398369/81‑1 (MQ=255)                                         gTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGt                                   >  2:398409/1‑81 (MQ=255)                                            aaCCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGtgc                                 <  2:398372/80‑1 (MQ=255)                                             aCCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGtgct                                <  1:398373/80‑1 (MQ=255)                                             aCCCGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGtgct                                <  1:398374/80‑1 (MQ=255)                                              cccGCTATCATTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGtgctgc                              >  2:398408/1‑81 (MQ=255)                                                       aTTACCGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTc                      <  2:398375/80‑1 (MQ=255)                                                           ccGTTTTCCTCCAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGc                  <  1:398376/80‑1 (MQ=255)                                                                 tcctccAGCGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGcc           >  1:398421/1‑81 (MQ=255)                                                                         cGGGTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTcg   <  2:398377/81‑1 (MQ=255)                                                                          gggTTCAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTcgc  >  2:398422/1‑81 (MQ=255)                                                                               |                                                                            ATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTTAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTCGC  >  NC_000913/2250746‑2250899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑