Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,134	T→A	N55Y (AAC→TAC)	psuK ←	putative pseudouridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,134	0	T	A	100.0%	67.8 / NA	20	N55Y (AAC→TAC)	psuK	putative pseudouridine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/9);  total (11/9)

CGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACC  >  NC_000913/2259056‑2259200                                                                               |                                                                   cGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAAc                                                                  >  2:399822/1‑81 (MQ=255)     aGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAg                                                              >  2:399820/1‑81 (MQ=255)                 ttGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGtt                                                  >  1:399825/1‑81 (MQ=255)                  tGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGtt                                                  <  2:399797/80‑1 (MQ=255)                          cagcGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCaa                                          >  1:399835/1‑80 (MQ=255)                          cagcGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAat                                         <  1:399798/81‑1 (MQ=255)                                ttGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTgcg                                   <  1:399799/81‑1 (MQ=255)                                    ccATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCt                               <  1:399800/81‑1 (MQ=255)                                      aTAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTAc                             <  1:399801/81‑1 (MQ=255)                                        aaaaaTCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTc                           >  1:399828/1‑81 (MQ=255)                                         aaaaTCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTc                           >  1:399837/1‑80 (MQ=255)                                         aaaaTCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTc                           >  1:399840/1‑80 (MQ=255)                                                 tGCCTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCg                  >  2:399833/1‑81 (MQ=255)                                                    cTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTaa               <  2:399802/81‑1 (MQ=255)                                                    cTACGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTa                >  1:399834/1‑80 (MQ=255)                                                      aCGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAat             >  1:399827/1‑81 (MQ=255)                                                      aCGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAat             >  2:399830/1‑81 (MQ=255)                                                       cGGCGCTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTaaa              <  2:399803/79‑1 (MQ=255)                                                         gcgcTCAGTAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAatttt          <  2:399804/81‑1 (MQ=255)                                                                 tAGCCAGGCTTTGTAACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTAcc  <  1:399805/81‑1 (MQ=255)                                                                               |                                                                   CGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGCCAGGCTTTGTTACCCAGCAACGCCAGGTTTTGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACC  >  NC_000913/2259056‑2259200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑