Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,779	T→C	K211R (AAA→AGA)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,779	0	T	C	100.0%	113.8 / NA	33	K211R (AAA→AGA)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

TAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCG  >  NC_000913/2260704‑2260856                                                                            |                                                                              tAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGcc                                                                           <  2:400081/80‑1 (MQ=255)   aGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCccgc                                                                        >  1:400116/1‑81 (MQ=255)   aGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCccg                                                                         <  2:400083/80‑1 (MQ=255)   aGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCccg                                                                         <  2:400082/80‑1 (MQ=255)      aaCGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCgg                                                                     >  2:400138/1‑81 (MQ=255)       aCGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGt                                                                    <  1:400084/81‑1 (MQ=255)             gTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATAc                                                              <  2:400086/81‑1 (MQ=255)             gTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATAc                                                              <  2:400085/81‑1 (MQ=255)             gTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATAc                                                              >  1:400141/1‑81 (MQ=255)                   aaGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGtt                                                        <  1:400087/81‑1 (MQ=255)                    aGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTg                                                       <  1:400088/81‑1 (MQ=255)                           gTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGc                                                <  1:400089/81‑1 (MQ=255)                           gTATGCGCCTGCACTCCCTTTCTTACTTTCAGTGGTCGTCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAg                                                 >  1:400142/1‑80 (MQ=255)                             aTGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCaa                                              >  2:400152/1‑81 (MQ=255)                             aTGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCaa                                              >  1:400146/1‑81 (MQ=255)                               gcgcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAAcc                                            >  1:400137/1‑81 (MQ=255)                                cgcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCg                                           >  1:400145/1‑81 (MQ=255)                                 gcCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGc                                          <  2:400090/81‑1 (MQ=255)                                           tctttcttACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGt                                >  1:400119/1‑81 (MQ=255)                                             tttcttACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTc                              >  1:400153/1‑81 (MQ=255)                                               tcttACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGtt                               <  1:400091/78‑1 (MQ=255)                                               tcttACTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTcc                             <  1:400092/80‑1 (MQ=255)                                                   aCTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTgcg                         <  1:400093/80‑1 (MQ=255)                                                    cTTTCAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGcgcg                       <  1:400094/81‑1 (MQ=255)                                                        cAGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGtt                    <  2:400095/80‑1 (MQ=255)                                                         aGTGGTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGtttt                  <  1:400096/81‑1 (MQ=255)                                                            ggTCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTttctt               >  2:400144/1‑81 (MQ=255)                                                              tCGCCGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCa             <  1:400097/81‑1 (MQ=255)                                                                gCCGTTTGAGCTCTGCCCGCCGGTTCATGCGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgc           <  1:400098/81‑1 (MQ=255)                                                                 ccGTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcg          >  2:400159/1‑81 (MQ=255)                                                                   gTTTGAGCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAgcgc         >  1:400139/1‑80 (MQ=255)                                                                         gCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCg  <  2:400099/81‑1 (MQ=255)                                                                         gCTCTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCg  <  1:400100/81‑1 (MQ=255)                                                                            |                                                                              TAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCG  >  NC_000913/2260704‑2260856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑