Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,837	T→C	intergenic (‑342/‑26)	fruB ← / → setB	fructose‑specific PTS multiphosphoryl transfer protein FruB/sugar exporter SetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,837	0	T	C	100.0%	87.6 / NA	26	intergenic (‑342/‑26)	fruB/setB	fructose‑specific PTS multiphosphoryl transfer protein FruB/sugar exporter SetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/10);  total (16/10)

AAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTC  >  NC_000913/2263760‑2263912                                                                              |                                                                            aaaTGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGcctt                                                                          <  1:400726/81‑1 (MQ=255) aaaTGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTCCTGACAAGCAGCGTAAACTCCGCGcctt                                                                          <  2:400727/81‑1 (MQ=255)     ggCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcctc                                                                      >  1:400758/1‑81 (MQ=255)      gCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcctct                                                                     >  1:400800/1‑81 (MQ=255)        aTTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcctcttc                                                                   <  1:400728/81‑1 (MQ=255)         ttttGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcctcttcc                                                                  <  2:400730/81‑1 (MQ=255)         ttttGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcctcttcc                                                                  <  2:400729/81‑1 (MQ=255)          tttGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGccttcctcttc                                                                   >  2:400771/1‑79 (MQ=255)               cTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGAt                                                            >  2:400763/1‑81 (MQ=255)               cTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGAt                                                            >  2:400756/1‑81 (MQ=255)                tATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATc                                                           >  1:400764/1‑81 (MQ=255)                 aTTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATc                                                           >  2:400773/1‑80 (MQ=255)                     gTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGAc                                                        >  1:400752/1‑79 (MQ=255)                         cgcTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAgcat                                                  <  1:400731/81‑1 (MQ=255)                          gcTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAgcatg                                                 <  1:400732/81‑1 (MQ=255)                                 aTTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACt                                          >  2:400760/1‑81 (MQ=255)                                 aTTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACt                                          >  2:400785/1‑81 (MQ=255)                                  tttCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTc                                         >  1:400770/1‑81 (MQ=255)                                  tttCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTc                                         <  2:400733/81‑1 (MQ=255)                                      gCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTccccc                                     >  2:400767/1‑81 (MQ=255)                                          tctgtTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCa                                  <  2:400734/80‑1 (MQ=255)                                             gtTTATTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGtct                              >  1:400762/1‑81 (MQ=255)                                                 aTTTTCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAg                          >  2:400784/1‑81 (MQ=255)                                                     tCTGACAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAgcgc                       >  1:400775/1‑80 (MQ=255)                                                         aCAAGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGaaa                   <  1:400735/80‑1 (MQ=255)                                                           aaGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCg                >  1:400766/1‑81 (MQ=255)                                                           aaGCAGCGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCg                >  1:400776/1‑81 (MQ=255)                                                              cagcGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGttt             <  1:400737/81‑1 (MQ=255)                                                              cagcGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGttt             <  1:400736/81‑1 (MQ=255)                                                               agcGTAAACTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGttt             >  2:400761/1‑80 (MQ=255)                                                                     aaCTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTtgacctga      >  1:400765/1‑81 (MQ=255)                                                                     aaCTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTtgacctg       <  2:400738/80‑1 (MQ=255)                                                                       cTCCGCGCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTtgacctgacc    <  1:400739/81‑1 (MQ=255)                                                                          cgcgCCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATTGTTTGACCTGACCTc  <  1:400740/80‑1 (MQ=255)                                                                              |                                                                            AAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTTATTTTCTGACAAGCAGCGTAAACTCCGCGTCTTCCTCTTCCAGTGATCGACCAGCATGCATAACTCCCCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTC  >  NC_000913/2263760‑2263912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑