Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,284,750	C→T	N125N (AAC→AAT)	yejM →	putative cardiolipin transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,284,750	0	C	T	100.0%	91.2 / NA	26	N125N (AAC→AAT)	yejM	putative cardiolipin transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

GATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTGGT  >  NC_000913/2284681‑2284827                                                                      |                                                                              gATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGaaaa                                                                    >  1:404511/1‑81 (MQ=255)          cGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGgc                                                           <  1:404475/81‑1 (MQ=255)                   tACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTg                                                  >  1:404539/1‑81 (MQ=255)                   tACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTg                                                  >  2:404524/1‑81 (MQ=255)                      tCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTgg                                                 >  2:404507/1‑79 (MQ=255)                       cGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAg                                              >  1:404518/1‑81 (MQ=255)                        gTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCTGACGAAAATGAGATGGCGCGCGACTGGCAGc                                             >  2:404509/1‑81 (MQ=255)                        gTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAg                                              <  2:404476/80‑1 (MQ=255)                        gTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAg                                              <  1:404477/80‑1 (MQ=255)                               tctccatctTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATg                                        <  2:404478/79‑1 (MQ=255)                                   catctTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTtcatc                                  <  1:404479/81‑1 (MQ=255)                                   catctTAATCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTtc                                     <  1:404480/78‑1 (MQ=255)                                         aaTCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGc                               <  1:404481/78‑1 (MQ=255)                                          aTCCCATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGc                           <  1:404482/81‑1 (MQ=255)                                             ccATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCg                         <  2:404483/80‑1 (MQ=255)                                              cATCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGt                       >  2:404523/1‑81 (MQ=255)                                               aTCGTCTGGCAACTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGt                       >  1:404540/1‑80 (MQ=255)                                                         aaCTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGc            >  1:404517/1‑81 (MQ=255)                                                           cTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCtt          <  1:404484/81‑1 (MQ=255)                                                           cTGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCtt          >  1:404520/1‑81 (MQ=255)                                                            tGGTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCtt          >  2:404546/1‑80 (MQ=255)                                                             ggTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTg         >  2:404519/1‑80 (MQ=255)                                                              gTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTg         <  2:404485/79‑1 (MQ=255)                                                              gTTATCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTg         <  1:404486/79‑1 (MQ=255)                                                                  tCAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTgg   >  1:404530/1‑81 (MQ=255)                                                                   cAATCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTGgt  >  1:404527/1‑81 (MQ=255)                                                                      |                                                                              GATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCGACTGGCAGCTGATGTTCATCAGCGTGCCGGTTATTTTATTGCTTGAACTGGT  >  NC_000913/2284681‑2284827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑