Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,313,525	C→T	S13L (TCA→TTA)	rcsD →	RcsD phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,313,525	0	C	T	100.0%	68.1 / NA	20	S13L (TCA→TTA)	rcsD	RcsD phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/12);  total (8/12)

CGCGAAGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGT  >  NC_000913/2313459‑2313600                                                                   |                                                                            cgcgAAGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCgggg                                                                <  2:410027/80‑1 (MQ=255)   cgAAGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGc                                                             <  2:410028/81‑1 (MQ=255)     aaGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCAt                                                           >  1:410053/1‑81 (MQ=255)          ttAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAGCGGCCACGGACCGCTTTTTACTCCTACCGGGGAGCAATAcc                                                       <  2:410029/80‑1 (MQ=255)            aaCAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGc                                                    >  2:410055/1‑81 (MQ=255)             aCAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGc                                                    <  1:410030/80‑1 (MQ=255)               aGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGcttc                                                 <  1:410031/81‑1 (MQ=255)                        acacGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTg                                        >  1:410056/1‑81 (MQ=255)                         cacGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGt                                       >  2:410054/1‑81 (MQ=255)                                     aGAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTgtgt                           <  2:410032/81‑1 (MQ=255)                                      gAAAGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTgtgt                           >  1:410067/1‑80 (MQ=255)                                        aaGAGACAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTAc                        >  1:410058/1‑81 (MQ=255)                                            gaCAACGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGt                    <  1:410033/81‑1 (MQ=255)                                               aaCGGCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGAc                 <  2:410034/81‑1 (MQ=255)                                                  ggCCACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGAt              <  2:410035/81‑1 (MQ=255)                                                     cACGACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATggg           >  2:410063/1‑81 (MQ=255)                                                        gACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGgtgt        <  1:410037/81‑1 (MQ=255)                                                        gACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGgtgt        <  2:410036/81‑1 (MQ=255)                                                        gACCCGCTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGgtg         >  2:410059/1‑80 (MQ=255)                                                              cTTTTTACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGt  <  1:410038/81‑1 (MQ=255)                                                                   |                                                                            CGCGAAGTTTTAACAGGTCATAAACACGAATGCGTCAGAAAGAGACAACGGCCACGACCCGCTTTTCACTCCTACCGGGGAGCATTACCCGCTTCTTTTTACTGTTGATCATTGTGTTACTGGTGACGATGGGTGTAATGGT  >  NC_000913/2313459‑2313600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑