Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,692	G→A	L36L (CTG→TTG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,692	0	G	A	100.0%	103.3 / NA	29	L36L (CTG→TTG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/15);  total (14/15)

ACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACG  >  NC_000913/2329618‑2329766                                                                           |                                                                           aCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATc                                                                      <  1:412993/81‑1 (MQ=255)    cccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGtg                                                                   >  2:413020/1‑81 (MQ=255)        aaCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGtgctgc                                                               >  2:413031/1‑81 (MQ=255)         aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGtgctgc                                                               <  1:412995/80‑1 (MQ=255)         aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGtgctg                                                                <  2:412994/79‑1 (MQ=255)         aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTgcg                                                              <  2:412996/81‑1 (MQ=255)          cGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTgcgc                                                             <  1:412997/81‑1 (MQ=255)              gtggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACcg                                                         >  2:413028/1‑81 (MQ=255)                 gtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACcgcg                                                       >  2:413026/1‑80 (MQ=255)                       gAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCAcc                                                <  2:412998/81‑1 (MQ=255)                       gAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCAc                                                 >  1:413038/1‑80 (MQ=255)                             ggTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGt                                          <  2:412999/81‑1 (MQ=255)                             ggTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGt                                          <  2:413000/81‑1 (MQ=255)                                 aTGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACg                                      >  1:413046/1‑81 (MQ=255)                                       agaCTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc                                 <  1:413002/80‑1 (MQ=255)                                       agaCTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGt                                  <  2:413001/79‑1 (MQ=255)                                         aCTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                              >  2:413054/1‑81 (MQ=255)                                         aCTTTGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                              <  1:413003/81‑1 (MQ=255)                                           tttGGTCACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcctc                            >  2:413039/1‑81 (MQ=255)                                                 cACGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAg                      >  2:413037/1‑81 (MQ=255)                                                  aCGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgt                     >  1:413055/1‑81 (MQ=255)                                                  aCGCCTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAg                      <  1:413004/80‑1 (MQ=255)                                                     ccTGATGACGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGtt                  <  1:413005/81‑1 (MQ=255)                                                        gatgaCGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg                >  2:413042/1‑80 (MQ=255)                                                         atgaCGACAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg                >  2:413067/1‑79 (MQ=255)                                                           gacgacAGCTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCa              >  1:413048/1‑79 (MQ=255)                                                                   cTGATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCaa    <  2:413006/81‑1 (MQ=255)                                                                     gATACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACg  >  2:413051/1‑81 (MQ=255)                                                                      aTACAATTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACg  <  1:413007/80‑1 (MQ=255)                                                                           |                                                                           ACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAACCAACG  >  NC_000913/2329618‑2329766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑