Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,342,381	C→T	V597I (GTT→ATT)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,342,381	0	C	T	100.0%	90.6 / NA	26	V597I (GTT→ATT)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

CCTGCCGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGC  >  NC_000913/2342305‑2342458                                                                             |                                                                              ccTGCCGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTgg                                                                           <  1:415518/81‑1 (MQ=255)  cTGCCGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGc                                                                          >  2:415561/1‑81 (MQ=255)        cATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTtat                                                                    <  2:415519/81‑1 (MQ=255)                cgcgTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTa                                                            <  2:415521/81‑1 (MQ=255)                cgcgTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTa                                                            <  2:415520/81‑1 (MQ=255)                        aaCGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAg                                                    >  1:415562/1‑81 (MQ=255)                            tCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGc                                                <  2:415522/81‑1 (MQ=255)                             cGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGc                                                >  2:415553/1‑80 (MQ=255)                               gAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAg                                             <  1:415523/81‑1 (MQ=255)                                aGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGt                                            >  2:415578/1‑81 (MQ=255)                                    aaTCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTc                                        >  2:415571/1‑81 (MQ=255)                                       ctcCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCt                                     >  2:415569/1‑81 (MQ=255)                                         ccATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCa                                   >  2:415563/1‑81 (MQ=255)                                          cATCACGCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCt                                     <  2:415524/78‑1 (MQ=255)                                                gCTTTCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAg                            <  2:415525/81‑1 (MQ=255)                                                  tttCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTTGGTATCTCCAAGCTGCGATTTTTCGCTCACCATCATCg                          >  1:415567/1‑81 (MQ=255)                                                   ttCTTGTTGCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCg                          >  2:415576/1‑80 (MQ=255)                                                           gCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAgg                 >  1:415575/1‑81 (MQ=255)                                                           gCTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAg                  <  1:415526/80‑1 (MQ=255)                                                            cTTATCGGTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGc                <  1:415527/81‑1 (MQ=255)                                                                  ggTAAAGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGAttt           <  1:415528/80‑1 (MQ=255)                                                                    aaaaGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATttgtg        >  2:415570/2‑81 (MQ=255)                                                                      aaGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATttgtgtt      <  1:415529/81‑1 (MQ=255)                                                                       aGATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATttgtgttg     <  2:415530/81‑1 (MQ=255)                                                                        gATAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATttgtgttgt    <  1:415531/81‑1 (MQ=255)                                                                          tAATCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGc  >  2:415605/1‑81 (MQ=255)                                                                             |                                                                              CCTGCCGCATCTGAACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGC  >  NC_000913/2342305‑2342458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑