Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,354,944	C→T	A227V (GCC→GTC)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,354,944	0	C	T	100.0%	120.5 / NA	34	A227V (GCC→GTC)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

CCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCT  >  NC_000913/2354868‑2355017                                                                             |                                                                          ccGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGAt                                                                       >  1:417950/1‑81 (MQ=255)    cTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGAc                                                                    <  1:417903/81‑1 (MQ=255)      gTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACa                                                                   >  1:417949/1‑80 (MQ=255)        tACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAAc                                                                >  1:417934/1‑81 (MQ=255)            tGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACtgtg                                                            >  1:417951/1‑81 (MQ=255)             gATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACtgt                                                             <  1:417904/79‑1 (MQ=255)              aTGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTgg                                                           <  2:417905/80‑1 (MQ=255)                 cgcTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgtt                                                       >  1:417943/1‑81 (MQ=255)                  gcTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgttg                                                      >  1:417941/1‑81 (MQ=255)                      aTTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgttggtt                                                   <  2:417906/80‑1 (MQ=255)                       ttCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCgttggttg                                                  <  2:417907/80‑1 (MQ=255)                           tGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTtgaatg                                              >  2:417947/1‑80 (MQ=255)                             tCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTtgaatgaa                                            >  1:417959/1‑80 (MQ=255)                             tCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTtgaatga                                             <  2:417908/79‑1 (MQ=255)                                    tACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTAcc                                    <  2:417909/81‑1 (MQ=255)                                          cGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGtt                               <  1:417912/80‑1 (MQ=255)                                          cGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTc                              <  1:417911/81‑1 (MQ=255)                                          cGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTc                              <  1:417910/81‑1 (MQ=255)                                                gATCCTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGAt                        >  1:417946/1‑81 (MQ=255)                                                    cTGATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCt                     <  1:417913/80‑1 (MQ=255)                                                      gATGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCtttt                  >  2:417944/1‑81 (MQ=255)                                                       aTGCCCGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCtttt                  <  2:417914/80‑1 (MQ=255)                                                          cccGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGcc               <  1:417916/80‑1 (MQ=255)                                                          cccGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGcc               <  1:417915/80‑1 (MQ=255)                                                           ccGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCaa             <  1:417917/81‑1 (MQ=255)                                                            cGCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAAc            >  1:417954/1‑81 (MQ=255)                                                             gCCTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACg           <  1:417918/81‑1 (MQ=255)                                                               cTGCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCt         >  1:417973/1‑81 (MQ=255)                                                                 gCTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgc       >  2:417948/1‑81 (MQ=255)                                                                  cTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgcc      <  2:417920/81‑1 (MQ=255)                                                                  cTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgc       <  1:417921/80‑1 (MQ=255)                                                                  cTTCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTg        <  1:417919/79‑1 (MQ=255)                                                                    tCGGTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgccgc    >  2:417956/1‑81 (MQ=255)                                                                      ggTCTGGTCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCt  <  2:417922/81‑1 (MQ=255)                                                                             |                                                                          CCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCT  >  NC_000913/2354868‑2355017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑