Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,061	T→C	F266S (TTT→TCT)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,061	0	T	C	100.0%	91.3 / NA	27	F266S (TTT→TCT)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

GTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAAC  >  NC_000913/2354986‑2355134                                                                            |                                                                          gTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTgc                                                                      >  2:417957/1‑81 (MQ=255)     aCTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGcc                                                                   <  2:417930/80‑1 (MQ=255)     aCTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCa                                                                  <  1:417929/81‑1 (MQ=255)        gATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAggg                                                               >  2:417971/1‑81 (MQ=255)           gCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTgg                                                            >  1:417958/1‑81 (MQ=255)               ttGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCgtg                                                        >  2:417968/1‑81 (MQ=255)                       gCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGcc                                                <  2:417931/81‑1 (MQ=255)                                  ccGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGa                                       <  1:417932/79‑1 (MQ=255)                                     tGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTg                                   >  1:417970/1‑80 (MQ=255)                                     tGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGa                                  <  1:417933/81‑1 (MQ=255)                                         gggCATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGaaaa                               >  2:417964/1‑80 (MQ=255)                                            cATTCGTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGt                           >  1:417961/1‑81 (MQ=255)                                                 gTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCt                      >  2:417974/1‑81 (MQ=255)                                                 gTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCt                      >  2:417969/1‑81 (MQ=255)                                                 gTCTGCAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCt                      <  2:417934/81‑1 (MQ=255)                                                      cAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTaaa                 <  2:417935/81‑1 (MQ=255)                                                      cAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTaa                  >  1:417975/1‑80 (MQ=255)                                                      cAAAACCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTa                   <  1:417936/79‑1 (MQ=255)                                                       aaaaCCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTaaaa                <  1:417937/81‑1 (MQ=255)                                                        aaaCCAGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTaaaaa               >  2:417980/1‑81 (MQ=255)                                                             aGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCg          >  1:417977/1‑81 (MQ=255)                                                             aGTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCg          <  1:417938/81‑1 (MQ=255)                                                              gTTACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGt         <  1:417939/81‑1 (MQ=255)                                                               ttACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTa        <  1:417940/81‑1 (MQ=255)                                                                tACAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAg       >  1:417972/1‑81 (MQ=255)                                                                 aCAGCGCCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGt      >  1:417976/1‑81 (MQ=255)                                                                      gcCAGTCTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAAc  >  2:417960/1‑80 (MQ=255)                                                                            |                                                                          GTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCATTCGTCTGCAAAACCAGTTACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAAC  >  NC_000913/2354986‑2355134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑