Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,359,162	C→T	A331T (GCG→ACG)	yfaV ←	putative transporter YfaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,359,162	0	C	T	100.0%	92.3 / NA	26	A331T (GCG→ACG)	yfaV	putative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/18);  total (8/18)

AGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGC  >  NC_000913/2359086‑2359238                                                                             |                                                                             aGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCAtt                                                                          <  2:418672/81‑1 (MQ=255)             aTCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAag                                                               >  1:418714/1‑80 (MQ=255)              tCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAagca                                                             <  2:418673/81‑1 (MQ=255)                gTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAagcagc                                                           >  2:418710/1‑81 (MQ=255)                   cAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGccc                                                         <  1:418674/80‑1 (MQ=255)                    aGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCg                                                        >  1:418722/1‑80 (MQ=255)                       acacTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGa                                                      >  2:418721/1‑79 (MQ=255)                        cacTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAg                                                   <  1:418675/81‑1 (MQ=255)                                   cGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaa                                        <  2:418676/81‑1 (MQ=255)                                    ggCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                       <  2:418677/81‑1 (MQ=255)                                     gCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATg                                      >  1:418695/1‑81 (MQ=255)                                       aaTAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                    <  2:418679/81‑1 (MQ=255)                                       aaTAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                    <  1:418678/81‑1 (MQ=255)                                         tAAACCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccg                                  >  2:418720/1‑81 (MQ=255)                                           aaCCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgcc                                >  1:418712/1‑81 (MQ=255)                                           aaCCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgcc                                <  2:418680/81‑1 (MQ=255)                                            aCCCGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgccg                               <  2:418681/81‑1 (MQ=255)                                             cccGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCcgccgc                              <  1:418682/81‑1 (MQ=255)                                              ccGATAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCgccgcc                             <  1:418683/81‑1 (MQ=255)                                                 aTAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                           >  2:418709/1‑80 (MQ=255)                                                 aTAGCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGt                          <  2:418684/81‑1 (MQ=255)                                                    gCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTaa                        <  1:418685/80‑1 (MQ=255)                                                    gCTGCAACACACAGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAAt                       <  2:418686/81‑1 (MQ=255)                                                             cacaGCGCTACGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCt              <  1:418687/81‑1 (MQ=255)                                                                       cGATCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACAtt      <  2:418688/79‑1 (MQ=255)                                                                         aTCGTCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGc  <  1:418689/81‑1 (MQ=255)                                                                             |                                                                             AGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGC  >  NC_000913/2359086‑2359238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑