Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,364,390	T→C	intergenic (‑115/‑164)	yfaZ ← / → nudI	putative porin YfaZ/pyrimidine deoxynucleotide diphosphatase NudI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,364,390	0	T	C	100.0%	35.8 / NA	12	intergenic (‑115/‑164)	yfaZ/nudI	putative porin YfaZ/pyrimidine deoxynucleotide diphosphatase NudI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

GGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAAATAATAATAAGTACA  >  NC_000913/2364320‑2364462                                                                       |                                                                         gggATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAACACTTCTTaaa                                                                >  1:419635/1‑81 (MQ=255)    aTTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAACACTTCTTAaataa                                                             >  2:419634/1‑81 (MQ=255)                       agGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATaa                                          <  2:419614/81‑1 (MQ=255)                               aTGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACtt                                  >  1:419633/1‑81 (MQ=255)                                     gAAAAAAAGGATCTAGTCACTTATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGa                            >  1:419627/1‑81 (MQ=255)                                       aaaaaaGGATCTAGTCACTTATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATa                          <  2:419615/81‑1 (MQ=255)                                        aaaaaGGATCTAGTCACTTATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATa                          >  1:419638/1‑80 (MQ=255)                                             ggATCTAGTCACTTATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATAcacaca                    <  2:419616/81‑1 (MQ=255)                                                    gTCACTTATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACaaaa                 <  1:419617/77‑1 (MQ=255)                                                         ttATTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAaataataat         >  2:419643/1‑80 (MQ=255)                                                           aTTGTAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAAATAATAATAAg      >  2:419642/1‑81 (MQ=255)                                                               tAACTAAACACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAAATAATAATAAGTACa  <  2:419618/81‑1 (MQ=255)                                                                       |                                                                         GGGATTGTACTGCCTGGCACAGAGGATTCAATGAGCGAAAAAAAGGATCTAGTCACTTATTGTAACTAAATACTTCTTAAATAATTAAATGCCGAAAAAATAAACACACTTTATCGATACACACAAAAATAATAATAAGTACA  >  NC_000913/2364320‑2364462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑