Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,372,742	G→C	P62P (CCG→CCC)	arnE →	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,372,742	0	G	C	100.0%	79.4 / NA	24	P62P (CCG→CCC)	arnE	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (8/16);  total (8/16)

TGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAA  >  NC_000913/2372671‑2372817                                                                        |                                                                            tgGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCAtt                                                                    <  2:421085/81‑1 (MQ=255) tgGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCAt                                                                     <  2:421084/80‑1 (MQ=255)   gCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTg                                                                   <  2:421086/80‑1 (MQ=255)   gCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGc                                                                  <  2:421087/81‑1 (MQ=255)       ggACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCtt                                                                <  2:421088/79‑1 (MQ=255)       ggACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTAc                                                              <  2:421089/81‑1 (MQ=255)                       gtcttggtcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTg                                               >  1:421126/1‑80 (MQ=255)                              tcttgCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGt                                       >  2:421136/1‑81 (MQ=255)                                  gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCt                                     <  1:421091/79‑1 (MQ=255)                                  gCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTg                                    <  1:421090/80‑1 (MQ=255)                                     aTGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGt                                 >  1:421139/1‑80 (MQ=255)                                          gCTCTGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgct                           >  1:421127/1‑81 (MQ=255)                                           ctctGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgct                           <  1:421092/80‑1 (MQ=255)                                            tctGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctgg                         >  2:421130/1‑81 (MQ=255)                                             ctGGCTGCTGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggc                        >  2:421141/1‑81 (MQ=255)                                                gctgctGGTCTTGCGGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggct                       <  1:421094/79‑1 (MQ=255)                                                gctgctGGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGc                     <  1:421093/81‑1 (MQ=255)                                                   gctgGTCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAg                   >  2:421135/1‑80 (MQ=255)                                                        tCTTGCAGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAAc             <  2:421096/81‑1 (MQ=255)                                                        tCTTGCAGAACGTACCCGTAGGCATTGCGTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGACTGCAGTAAAAc             <  2:421095/81‑1 (MQ=255)                                                              aGAACGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGc       <  2:421097/81‑1 (MQ=255)                                                                 aCGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACg    <  1:421098/81‑1 (MQ=255)                                                                  cGTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGa   <  2:421099/81‑1 (MQ=255)                                                                   gTACCCGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGaa  >  1:421140/1‑81 (MQ=255)                                                                        |                                                                            TGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAA  >  NC_000913/2372671‑2372817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑