Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,384,163	T→C	V520V (GTA→GTG)	yfbK ←	IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,384,163	0	T	C	100.0%	78.5 / NA	24	V520V (GTA→GTG)	yfbK	IPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/14);  total (10/14)

GAGCCCACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGACTTT  >  NC_000913/2384087‑2384239                                                                             |                                                                             gAGCCCACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCActg                                                                          >  2:423267/1‑81 (MQ=255) gAGCCCACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCActg                                                                          >  1:423265/1‑81 (MQ=255)  aGCCCACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCActgc                                                                         >  2:423272/1‑81 (MQ=255)     ccACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACtgctgc                                                                      >  1:423270/1‑81 (MQ=255)           tttGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGaaa                                                                <  2:423244/81‑1 (MQ=255)           tttGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGa                                                                  <  1:423243/79‑1 (MQ=255)                 ctgctgCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCa                                                           >  2:423269/1‑80 (MQ=255)                  tgctgcCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCata                                                         >  2:423275/1‑81 (MQ=255)                    ctgcCAGGAGGTATTGTTCGGGTATTCAGAACCGCGTAACTTTTGCCCATGTGCAGCCACTGCTGCGCGAAAACGCat                                                          <  1:423246/78‑1 (MQ=255)                    ctgcCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGc                                                            <  2:423245/76‑1 (MQ=255)                       ccAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCtt                                                     <  2:423247/80‑1 (MQ=255)                                   gTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGt                                         <  2:423248/80‑1 (MQ=255)                                       aGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTAtt                                    <  1:423250/81‑1 (MQ=255)                                       aGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTAtt                                    <  1:423249/81‑1 (MQ=255)                                                gAACCGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGcccc                           <  1:423251/81‑1 (MQ=255)                                                   ccGCGTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGc                        <  2:423252/81‑1 (MQ=255)                                                     gcgTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCgg                      >  2:423278/1‑81 (MQ=255)                                                      cgTAACTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCggg                     <  1:423253/81‑1 (MQ=255)                                                           cTTTTGCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTc                <  2:423254/81‑1 (MQ=255)                                                                gCCCATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCaact            <  2:423255/80‑1 (MQ=255)                                                                   cATATGCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCaactaact        <  2:423256/81‑1 (MQ=255)                                                                        gCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGActt   >  1:423281/1‑81 (MQ=255)                                                                        gCAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGActt   >  1:423273/1‑81 (MQ=255)                                                                         cAGCCACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGActtt  >  1:423280/1‑81 (MQ=255)                                                                             |                                                                             GAGCCCACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGACTTT  >  NC_000913/2384087‑2384239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑