Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,386,436	G→A	A193T (GCA→ACA)	yfbL →	putative peptidase YfbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,386,436	0	G	A	100.0%	55.9 / NA	18	A193T (GCA→ACA)	yfbL	putative peptidase YfbL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

TCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTT  >  NC_000913/2386369‑2386511                                                                    |                                                                            tCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAgat                                                                   <  2:423566/78‑1 (MQ=255)            aaaTGGGGAGCGCGGTGCATGCAGCTTCCCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTAc                                                      >  2:423603/1‑80 (MQ=255)               tGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGAct                                                  <  2:423567/81‑1 (MQ=255)                   gAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTgc                                              >  1:423597/1‑81 (MQ=255)                    aGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTgcg                                             >  2:423623/1‑81 (MQ=255)                                 caGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGAATCTGCGCCTGGAAGCCAg                                 <  2:423568/80‑1 (MQ=255)                                   gCTTCGCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAAt                              >  1:423607/1‑81 (MQ=255)                                        gCTTGAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAAttaccc                         >  1:423605/1‑81 (MQ=255)                                            gAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAAttacccttac                     >  1:423612/1‑81 (MQ=255)                                            gAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAAttaccctt                       <  2:423569/79‑1 (MQ=255)                                            gAGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAAttaccctt                       <  1:423570/79‑1 (MQ=255)                                             aGCGTCCAGTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAAttacccttac                     <  1:423571/80‑1 (MQ=255)                                                     gTGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATg            <  2:423572/81‑1 (MQ=255)                                                      tGAAATTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGt           >  2:423611/1‑81 (MQ=255)                                                         aaTTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCt        >  1:423574/1‑81 (MQ=255)                                                         aaTTAATGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCt        <  1:423573/81‑1 (MQ=255)                                                             aaTGATAACACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCt    >  1:423610/1‑81 (MQ=255)                                                               tGATAACACTGGAGATGATTGGCTAGTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCttt  <  2:423575/81‑1 (MQ=255)                                                                    |                                                                            TCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTT  >  NC_000913/2386369‑2386511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑