Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,450,781	T→C	T99A (ACT→GCT)	yfcQ ←	putative fimbrial protein YfcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,450,781	0	T	C	100.0%	98.8 / NA	29	T99A (ACT→GCT)	yfcQ	putative fimbrial protein YfcQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/6);  total (23/6)

CGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGG  >  NC_000913/2450703‑2450855                                                                               |                                                                           cGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGctt                                                                          <  2:436019/81‑1 (MQ=255)     gATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAAt                                                                      >  2:436041/1‑81 (MQ=255)     gATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAAt                                                                      >  1:436046/1‑81 (MQ=255)         gCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATtgct                                                                  <  1:436020/81‑1 (MQ=255)         gCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATtgct                                                                  <  2:436021/81‑1 (MQ=255)         gCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATtgct                                                                  >  1:436056/1‑81 (MQ=255)           aaGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATtgctg                                                                 >  1:436043/1‑80 (MQ=255)                  gTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCAtt                                                          >  1:436051/1‑80 (MQ=255)                   tATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTaa                                                        <  2:436022/81‑1 (MQ=255)                    aTTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTaa                                                        >  1:436042/1‑80 (MQ=255)                         gTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTgg                                                  <  2:436023/81‑1 (MQ=255)                            tAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGttt                                               >  2:436054/1‑81 (MQ=255)                                aCGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTtgtg                                            >  1:436059/1‑80 (MQ=255)                                aCGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTtgtg                                            >  1:436062/1‑80 (MQ=255)                                aCGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTgtgt                                           <  2:436024/81‑1 (MQ=255)                                  gCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTAc                                         >  2:436047/1‑81 (MQ=255)                                   cTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTAc                                         >  2:436060/1‑80 (MQ=255)                                   cTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTAc                                         >  2:436048/1‑80 (MQ=255)                                      aCCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGt                                     >  1:436045/1‑81 (MQ=255)                                         aTCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTc                                    >  1:436055/1‑79 (MQ=255)                                            gTGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGt                               >  1:436053/1‑81 (MQ=255)                                             tGCTGAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGt                               >  1:436044/1‑80 (MQ=255)                                                 gAAGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAggg                          >  2:436050/1‑81 (MQ=255)                                                  aaGCTCGATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGt                         <  2:436025/81‑1 (MQ=255)                                                        gATACCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCAtctc                   >  2:436066/1‑81 (MQ=255)                                                           aCCAAAACCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTccc                 >  1:436065/1‑80 (MQ=255)                                                                aaCCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTg           >  2:436063/1‑81 (MQ=255)                                                                aaCCGGGTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTg           >  2:783667/1‑81 (MQ=255)                                                                     ggTACATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGa      >  1:436052/1‑81 (MQ=255)                                                                         cATCAGCTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACggg  >  2:436058/1‑81 (MQ=255)                                                                               |                                                                           CGTTGATAGCAAGCGGAGTATTGAGTTTAAAACGCTGACCATCGTGCTGAAGCTCGATACCAAAACCGGGTACATCAGTTTCAATTGCTGCATCATTAAAACTGGTTTGTGTACCAGTCCAGGTGAGGGTCATCTCCCAATCTGGATGACGGG  >  NC_000913/2450703‑2450855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑