Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,451,016	A→G	D20D (GAT→GAC)	yfcQ ←	putative fimbrial protein YfcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,451,016	0	A	G	100.0%	106.4 / NA	31	D20D (GAT→GAC)	yfcQ	putative fimbrial protein YfcQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/13);  total (18/13)

CGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCAC  >  NC_000913/2450940‑2451089                                                                             |                                                                          cGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcag                                                                       >  1:436087/1‑81 (MQ=255)  gACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcag                                                                       >  1:436103/1‑80 (MQ=255)   aCTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcagca                                                                     <  2:436053/81‑1 (MQ=255)   aCTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTcagca                                                                     <  2:436052/81‑1 (MQ=255)        aaTGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATg                                                                <  1:436054/81‑1 (MQ=255)                 accaccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATgg                                                        <  2:436055/80‑1 (MQ=255)                 accaccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgc                                                       >  1:436125/1‑81 (MQ=255)                   caccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgcgc                                                     >  2:436084/1‑81 (MQ=255)                   caccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgcgc                                                     >  2:436101/1‑81 (MQ=255)                   caccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGgcgc                                                     >  2:436091/1‑81 (MQ=255)                     ccGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTa                                                   <  2:436056/81‑1 (MQ=255)                       gCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACt                                                 <  1:436057/81‑1 (MQ=255)                          gATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGa                                               >  2:436109/1‑80 (MQ=255)                           aTGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGaca                                             >  1:436090/1‑81 (MQ=255)                           aTGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGaca                                             <  1:436058/81‑1 (MQ=255)                             gCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGacac                                            >  1:436096/1‑80 (MQ=255)                              cTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACaa                                          <  2:436059/81‑1 (MQ=255)                                 cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAg                                        >  2:436097/1‑80 (MQ=255)                                 cAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAg                                        >  1:436092/1‑80 (MQ=255)                                          ggTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGtt                                 >  1:436098/1‑78 (MQ=255)                                          ggTGACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGtt                                 >  1:436093/1‑78 (MQ=255)                                             gACAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGa                             >  1:436102/1‑79 (MQ=255)                                              aCAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAg                          >  1:436110/1‑81 (MQ=255)                                               cAATAATGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGt                         >  1:436104/1‑81 (MQ=255)                                                    atGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCttt                     <  1:436060/80‑1 (MQ=255)                                                    atGTCCCGTGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTtc                    <  2:436061/81‑1 (MQ=255)                                                            tGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGAt            <  2:436062/81‑1 (MQ=255)                                                            tGAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGAt            <  1:436063/81‑1 (MQ=255)                                                             gAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGa             >  1:436100/1‑79 (MQ=255)                                                             gAAAGGTGATGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGa             >  2:436115/1‑79 (MQ=255)                                                                      tGTCTTCGTCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTccaccac  <  2:436064/81‑1 (MQ=255)                                                                             |                                                                          CGACTTCAATGGTTTTACCACCGCTGATGCTACAGGTTGGCGGTGACAATAATGTCCCGTGAAAGGTGATGTCTTCATCAGCAGCATGAGCTATGGCGCTACTGACACAAAGCAGGGTTAAGAAAGTCTTTCTCATTGATAGTCCACCAC  >  NC_000913/2450940‑2451089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑