Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,451,787	C→T	A184T (GCC→ACC)	yfcS ←	putative fimbrial chaperone YfcS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,451,787	0	C	T	100.0%	76.9 / NA	22	A184T (GCC→ACC)	yfcS	putative fimbrial chaperone YfcS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/15);  total (7/15)

GTTTTCGGCTCAATGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGCGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCTGCGGGTGAGCGTCACCTT  >  NC_000913/2451723‑2451862                                                                 |                                                                            gTTTTCGGCTCAATGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCAc                                                              <  1:436195/80‑1 (MQ=255)   tttCGGCTCAATGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACg                                                             >  1:436226/1‑79 (MQ=255)        gCTCAATGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACg                                                      <  2:436196/81‑1 (MQ=255)         cTCAATGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACgg                                                     <  1:436197/81‑1 (MQ=255)            aaTGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCg                                                   <  1:436198/80‑1 (MQ=255)             aTGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGtt                                                 <  2:436199/81‑1 (MQ=255)                  cAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGtt                                            <  2:436200/81‑1 (MQ=255)                     agGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTAtt                                         <  2:436201/81‑1 (MQ=255)                            aaCCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGt                                   >  2:436240/1‑80 (MQ=255)                            aaCCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTa                                  <  2:436202/81‑1 (MQ=255)                               ccTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAAt                               <  2:436203/81‑1 (MQ=255)                                cTGCAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATg                              >  2:436247/1‑81 (MQ=255)                                   cAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGcc                            <  2:436205/80‑1 (MQ=255)                                   cAGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGc                             <  1:436204/79‑1 (MQ=255)                                    aGCTGGATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCt                           >  1:436238/1‑80 (MQ=255)                                         gATTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGc                     >  2:436235/1‑81 (MQ=255)                                          aTTACCGTTTTTCTGCGCACTGGTGTTGCTGATAATCACGTATTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCt                    <  1:436206/81‑1 (MQ=255)                                                  ttttCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCTGCGGGt              >  2:436243/1‑79 (MQ=255)                                                     tCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCTGCGGGTGAGc          <  2:436207/80‑1 (MQ=255)                                                     tCTGCGCACTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCTGCGGGTGAGCg         >  1:436245/1‑81 (MQ=255)                                                            aCTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCTGCGGGTGAGCGTCACCtt  <  1:436209/81‑1 (MQ=255)                                                            aCTGGTGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCTGCGGGTGAGCGTCACCtt  <  1:436208/81‑1 (MQ=255)                                                                 |                                                                            GTTTTCGGCTCAATGACCAGAGGCGAGAACCCTGCAGCTGGATTACCGTTTTTCTGCGCACTGGCGTTGCTGATAATCACGTAGTACGGCGTTGGGTTATTGACGGTAAATGCCTGACCGCTGCGGGTGAGCGTCACCTT  >  NC_000913/2451723‑2451862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑