| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,483,224 | A→G | L39S (TTA→TCA) | emrK ← | tripartite efflux pump membrane fusion protein EmrK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,483,224 | 0 | A | G | 94.1% | 47.9 / ‑3.6 | 17 | L39S (TTA→TCA) | emrK | tripartite efflux pump membrane fusion protein EmrK |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (6/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATT > NC_000913/2483155‑2483296 | gagaCTTGTGCAGAAATTGGATCTGCATTCCACGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGAc > 1:441194/1‑81 (MQ=255) agaCTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGAc > 1:441209/1‑80 (MQ=255) cAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAataggcat < 1:441176/81‑1 (MQ=255) gATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCt < 1:441177/80‑1 (MQ=255) cATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGa < 1:441178/76‑1 (MQ=255) ccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataa < 2:441179/81‑1 (MQ=255) gTGACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat < 2:441180/81‑1 (MQ=255) tGACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat > 2:441207/1‑80 (MQ=255) gACATAGGCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata > 1:441204/1‑80 (MQ=255) gCGTCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAcc > 2:441208/1‑81 (MQ=255) tCATCTGTACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc < 2:441181/81‑1 (MQ=255) tACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt < 2:441182/77‑1 (MQ=255) tACTAATCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt < 1:441183/77‑1 (MQ=255) aaTCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaa > 2:441205/1‑79 (MQ=255) aTCATGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaaa < 1:441184/80‑1 (MQ=255) tGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAtt < 1:441186/81‑1 (MQ=255) tGTCTTCTGATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAtt < 1:441185/81‑1 (MQ=255) | GAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATT > NC_000913/2483155‑2483296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |