Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	273,644	G→A	A266A (GCG→GCA)	ykfC →	CP4‑6 prophage; PF00078 domain‑containing protein YkfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	273,644	0	G	A	100.0%	102.3 / NA	29	A266A (GCG→GCA)	ykfC	CP4‑6 prophage; PF00078 domain‑containing protein YkfC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

GAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGG  >  NC_000913/273567‑273721                                                                              |                                                                              gAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGt                                                                             >  1:63456/1‑80 (MQ=255)  aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTc                                                                            <  2:63420/80‑1 (MQ=255)  aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTc                                                                            <  2:63421/80‑1 (MQ=255)         ccTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGaaaa                                                                    <  1:63423/81‑1 (MQ=255)         ccTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGaaaa                                                                    <  2:63422/81‑1 (MQ=255)          cTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAAc                                                                   >  2:63467/1‑81 (MQ=255)           tGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACt                                                                  >  2:63484/1‑81 (MQ=255)              gCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGc                                                               <  1:63424/81‑1 (MQ=255)                    aaGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGa                                                         <  1:63425/81‑1 (MQ=255)                     aGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGaa                                                        <  1:63426/81‑1 (MQ=255)                     aGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGa                                                         >  1:63464/1‑80 (MQ=255)                          gAAAAGATCGGTGGTAATGGAATAACAGTAACCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCcg                                                   <  1:63427/81‑1 (MQ=255)                            aaaGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCcgcg                                                 >  2:63491/1‑81 (MQ=255)                                 tCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGc                                            <  1:63428/81‑1 (MQ=255)                                   ggtggtACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCg                                           <  1:63429/80‑1 (MQ=255)                                      ggtACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACt                                       >  1:63497/1‑81 (MQ=255)                                       gtACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTg                                      >  1:63496/1‑81 (MQ=255)                                             gAATAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCt                                 <  1:63430/80‑1 (MQ=255)                                               aTAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATg                              <  2:63432/81‑1 (MQ=255)                                               aTAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATg                              <  1:63431/81‑1 (MQ=255)                                                tAACAGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGc                             <  1:63433/81‑1 (MQ=255)                                                    aGTATCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCgat                         >  2:63471/1‑81 (MQ=255)                                                       aTCCAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCgatgat                      >  2:63482/1‑81 (MQ=255)                                                          cAACGGGGCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtttt                   <  2:63434/81‑1 (MQ=255)                                                               gggCCGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCt              >  2:63492/1‑81 (MQ=255)                                                                  ccGAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCAt           <  1:63435/81‑1 (MQ=255)                                                                    gAAGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCg         >  1:63476/1‑81 (MQ=255)                                                                     aaGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGt        >  2:63474/1‑81 (MQ=255)                                                                      aGTACGGCAGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGt        >  2:63481/1‑80 (MQ=255)                                                                           ggcagTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAgg  >  1:63483/1‑81 (MQ=255)                                                                              |                                                                              GAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGG  >  NC_000913/273567‑273721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑