Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,489,635	G→A	I184I (ATC→ATT)	yfdV ←	putative transport protein YfdV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,489,635	0	G	A	100.0%	70.3 / NA	21	I184I (ATC→ATT)	yfdV	putative transport protein YfdV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/14);  total (7/14)

ACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTA  >  NC_000913/2489558‑2489704                                                                              |                                                                      aCCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATc                                                                    >  1:442071/1‑81 (MQ=255)  ccccTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCg                                                                   <  1:442037/81‑1 (MQ=255)  ccccTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCg                                                                   <  2:442036/81‑1 (MQ=255)             gCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAAc                                                         <  1:442038/80‑1 (MQ=255)                  cGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGt                                                     <  1:442039/79‑1 (MQ=255)                  cGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGc                                                   >  1:442069/1‑81 (MQ=255)                     aaTCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCa                                                <  2:442040/81‑1 (MQ=255)                       tCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCAta                                              <  2:442041/81‑1 (MQ=255)                          gATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCAtacta                                           >  1:442070/1‑81 (MQ=255)                                aGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCt                                     <  1:442042/81‑1 (MQ=255)                                     gggTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCtt                                 <  2:442043/80‑1 (MQ=255)                                      ggTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCttt                                <  1:442044/80‑1 (MQ=255)                                       gTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGc                              >  1:442067/1‑81 (MQ=255)                                        tCCCATGCTGCCGGAATTTTTACCCCAACCAGCACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGcc                             <  2:442045/81‑1 (MQ=255)                                                 gcCGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAAt                     >  2:442072/1‑80 (MQ=255)                                                   cGGAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTaa                  <  2:442046/81‑1 (MQ=255)                                                    ggAATTTTTACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAAt                 <  2:442047/81‑1 (MQ=255)                                                           ttACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTc           <  2:442048/80‑1 (MQ=255)                                                            tACCCCAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCt            >  2:442073/1‑78 (MQ=255)                                                              ccccAACCAACACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGa        <  2:442049/80‑1 (MQ=255)                                                                   accaacACCAGAATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTa  >  2:442068/1‑81 (MQ=255)                                                                              |                                                                      ACCCCTGAGTTAGCCTTCGCAATCAGATTAAAGGTTGGGTCCCATGCTGCCGGAATTTTTACCCCAACCAACACCAGGATCGTTGCCAGAACAGGTGCCCATACTACTGGCTCCTTTGCCGCAGAAATTAATGCGCTCAGATTACTA  >  NC_000913/2489558‑2489704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑