Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,498,790	C→T	H40H (CAC→CAT)	ypdA →	sensory histidine kinase YpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,498,790	0	C	T	100.0%	110.9 / NA	31	H40H (CAC→CAT)	ypdA	sensory histidine kinase YpdA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

GGCAGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCC  >  NC_000913/2498712‑2498864                                                                               |                                                                           ggCAGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTc                                                                          >  1:443460/1‑81 (MQ=255) ggCAGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTc                                                                          >  1:443469/1‑81 (MQ=255)    aGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTcccc                                                                       <  1:443438/81‑1 (MQ=255)         tAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCaa                                                                     >  1:443467/1‑78 (MQ=255)           aTGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGaa                                                                >  2:443486/1‑81 (MQ=255)              cTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAAtt                                                              <  2:443439/80‑1 (MQ=255)                     gCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCg                                                      >  1:443473/1‑81 (MQ=255)                             tttctCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGc                                               >  1:443464/1‑80 (MQ=255)                              ttctCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCa                                             >  1:443465/1‑81 (MQ=255)                                ctcATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCAtt                                           >  2:443466/1‑81 (MQ=255)                                  cATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCAtttt                                         >  2:443476/1‑81 (MQ=255)                                    tCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTtct                                       <  2:443440/81‑1 (MQ=255)                                      cGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTtctc                                      <  2:443441/80‑1 (MQ=255)                                            cGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGtt                                <  2:443442/80‑1 (MQ=255)                                            cGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTc                               <  2:443443/81‑1 (MQ=255)                                               cTGTTTCGCGAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGcc                            >  2:443511/1‑81 (MQ=255)                                                     cgcgAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGtt                       <  1:443445/80‑1 (MQ=255)                                                     cgcgAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGtt                       <  1:443444/80‑1 (MQ=255)                                                      gcgAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTc                      <  1:443446/80‑1 (MQ=255)                                                        gAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAg                    >  1:443470/1‑80 (MQ=255)                                                        gAACTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAg                    >  2:443479/1‑80 (MQ=255)                                                          aCTGTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCAc                 <  1:443447/81‑1 (MQ=255)                                                             gTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTg              >  1:443480/1‑81 (MQ=255)                                                             gTTGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTg              >  2:443483/1‑81 (MQ=255)                                                              ttGCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTgg             >  1:443468/1‑81 (MQ=255)                                                                gCACAAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGt            <  2:443448/80‑1 (MQ=255)                                                                  acaAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCg         >  1:443477/1‑81 (MQ=255)                                                                  acaAGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCg         <  2:443449/81‑1 (MQ=255)                                                                    aaGTCGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGc       <  2:443450/81‑1 (MQ=255)                                                                        cGGCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTc   >  2:443489/1‑81 (MQ=255)                                                                         ggCGCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTcc  >  2:443492/1‑81 (MQ=255)                                                                               |                                                                           GGCAGCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCC  >  NC_000913/2498712‑2498864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑