Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,520,945	G→A	noncoding (15/76 nt)	valU →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,520,945	0	G	A	100.0%	58.8 / NA	18	noncoding (15/76 nt)	valU	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

TAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATG  >  NC_000913/2520875‑2521017                                                                       |                                                                         tAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGgag                                                                   <  2:447814/78‑1 (MQ=255) tAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGagag                                                                 >  1:447847/1‑80 (MQ=255)          cGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCtt                                                       <  2:447815/81‑1 (MQ=255)           gTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTa                                                      >  1:447840/1‑81 (MQ=255)              gACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTAc                                                     >  1:447849/1‑79 (MQ=255)                   aTTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAaggagg                                              >  2:447839/1‑81 (MQ=255)                         aaCTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTc                                         <  1:447816/80‑1 (MQ=255)                          aCTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGAGGGTcgg                                       <  2:447817/81‑1 (MQ=255)                           ctctCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggc                                      >  2:447842/1‑81 (MQ=255)                               cccTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                                  >  2:447843/1‑81 (MQ=255)                                ccTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTc                                 <  1:447818/81‑1 (MQ=255)                                    taATGCGACTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                             <  2:447819/81‑1 (MQ=255)                                           aCTCCACACAGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                      <  2:447820/81‑1 (MQ=255)                                                acacaGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacc                  >  2:447844/1‑80 (MQ=255)                                                  acaGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacccac               <  2:447821/81‑1 (MQ=255)                                                    aGCGGGGGTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacccacc              >  2:447845/1‑80 (MQ=255)                                                       gggggTGATTAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggc                                      >  1:447853/1‑53 (MQ=16)                                                                tAGCTCAACTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATg  >  2:447846/1‑80 (MQ=255)                                                                       |                                                                         TAGAAAATGCGTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATG  >  NC_000913/2520875‑2521017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑