Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,532,981	C→T	G191G (GGC→GGT)	cysK →	O‑acetylserine sulfhydrylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,532,981	0	C	T	100.0%	104.1 / NA	30	G191G (GGC→GGT)	cysK	O‑acetylserine sulfhydrylase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

GACGGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATCGCCCAGGCGCTGGCAGG  >  NC_000913/2532904‑2533058                                                                              |                                                                              gACGGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTAca                                                                            <  1:450135/81‑2 (MQ=255) gACGGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTAc                                                                             >  1:450171/1‑80 (MQ=255) gACGGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTAc                                                                             >  1:450172/1‑80 (MQ=255)  aCGGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTAcc                                                                            >  2:450167/1‑80 (MQ=255)  aCGGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTAcc                                                                            >  1:450179/1‑80 (MQ=255)      tCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAgg                                                                       <  1:450136/81‑1 (MQ=255)          gTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGc                                                                      >  1:450176/1‑78 (MQ=255)          gTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAg                                                                   <  1:450137/81‑1 (MQ=255)           ttGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGa                                                                  <  1:450138/81‑1 (MQ=255)                gTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGAt                                                             >  2:450168/1‑81 (MQ=255)                 tatttattGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATc                                                            <  1:450139/81‑1 (MQ=255)                     tattGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTAt                                                        >  2:450173/1‑81 (MQ=255)                             gCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTc                                                 <  2:450141/80‑1 (MQ=255)                             gCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTc                                                 <  2:450140/80‑1 (MQ=255)                                     aCTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAg                                        <  2:450142/81‑1 (MQ=255)                                         gCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGcc                                      <  2:450143/79‑1 (MQ=255)                                         gCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCa                                     <  1:450144/80‑1 (MQ=255)                                           ggTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAAcc                                  >  2:450189/1‑81 (MQ=255)                                            gTACGCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCg                                 <  1:450145/81‑1 (MQ=255)                                                gCTGACTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATtc                             >  2:450175/1‑81 (MQ=255)                                                    aCTGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCa                         <  2:450146/81‑1 (MQ=255)                                                      tGGCGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGt                       >  1:450185/1‑81 (MQ=255)                                                         cGTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTAt                    >  1:450177/1‑81 (MQ=255)                                                          gTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATc                   >  2:450178/1‑81 (MQ=255)                                                          gTCAGCCGCTACATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATc                   >  2:450190/1‑81 (MQ=255)                                                                    aCATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATCGCCCAGgcgc         >  2:450182/1‑81 (MQ=255)                                                                     cATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATCGCCCAGgcg          <  2:450148/79‑1 (MQ=255)                                                                     cATTAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATCGCCCAGGCGCt        <  1:450147/81‑1 (MQ=255)                                                                        tAAAGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATCGCCCAGGCGCTgg      >  2:450186/1‑80 (MQ=255)                                                                           aGGTACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATCGCCCAGGCGCTGGCAgg  <  2:450149/81‑1 (MQ=255)                                                                              |                                                                              GACGGTCAGGTTGATGTATTTATTGCTGGCGTTGGGACTGGCGGTACGCTGACTGGCGTCAGCCGCTACATTAAAGGCACCAAAGGCAAGACCGATCTTATCTCTGTCGCCGTTGAGCCAACCGATTCTCCAGTTATCGCCCAGGCGCTGGCAGG  >  NC_000913/2532904‑2533058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑