Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,575,156	G→T	T101K (ACG→AAG)	eutP ←	putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,575,156	0	G	T	100.0%	80.9 / NA	23	T101K (ACG→AAG)	eutP	putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/14);  total (9/14)

GCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCGGCAGGCAACCGACTTTCC  >  NC_000913/2575079‑2575227                                                                              |                                                                        gCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCtttt                                                                      >  2:458779/1‑81 (MQ=255) gCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCtttt                                                                      <  2:458739/81‑1 (MQ=255)  cTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTtttt                                                                     <  1:458740/81‑1 (MQ=255)        cATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACtgat                                                               >  2:458782/1‑81 (MQ=255)           cGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGAc                                                            >  2:458777/1‑81 (MQ=255)             gCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACgg                                                          <  1:458741/81‑1 (MQ=255)             gCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACgg                                                          >  2:458780/1‑81 (MQ=255)                   cGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGAtt                                                     <  2:458742/80‑1 (MQ=255)                   cGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGAtt                                                     <  1:458743/80‑1 (MQ=255)                    gAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGAttt                                                    <  1:458744/80‑1 (MQ=255)                    gAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTg                                                   >  2:458783/1‑81 (MQ=255)                      aaCCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTgtc                                                 >  2:458785/1‑79 (MQ=255)                                aaGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGAc                                       <  1:458745/81‑1 (MQ=255)                                       aaCTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAata                                >  1:458789/1‑81 (MQ=255)                                            tCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCa                            <  1:458746/80‑1 (MQ=255)                                               cgTTGCGGCGACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCaa                        <  1:458747/81‑1 (MQ=255)                                                    cggcgACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCgg                   <  1:458748/81‑1 (MQ=255)                                                     ggcgACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCggc                  <  2:458749/81‑1 (MQ=255)                                                     ggcgACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCggc                  <  2:458750/81‑1 (MQ=255)                                                      gcgACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCggca                 <  2:458751/81‑1 (MQ=255)                                                        gACGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCggcagg               >  2:458781/1‑81 (MQ=255)                                                         aCGTCAGCATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCggcagg               >  1:458788/1‑80 (MQ=255)                                                                cATCTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCGGCAGGCAACCGAc       <  2:458752/81‑1 (MQ=255)                                                                   cTGGCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCGGCAGGCAACCGACttt    >  2:458784/1‑81 (MQ=255)                                                                      gCATGTCCTTTTTACTGATGACGGCGATTTGTCGTTTGCTGACGCCAATATCCAGCAACCCGGCAGGCAACCGACTTTcc  <  1:458753/80‑1 (MQ=255)                                                                              |                                                                        GCTCGAACATCGGCTCTTCGAAACCCGTCTCAAGCAATAACTTTCGCGTTGCGGCGACGTCAGCATCTGGCATGTCCGTTTTACTGATGACGGCGATTTGCCGTTTGCTGACGCCAATATCCAGCAACCCGGCAGGCAACCGACTTTCC  >  NC_000913/2575079‑2575227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑