Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,419	C→T	Q252* (CAG→TAG)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,419	0	C	T	100.0%	89.2 / NA	26	Q252* (CAG→TAG)	talA	transaldolase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/10);  total (16/10)

TCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGA  >  NC_000913/2579342‑2579495                                                                              |                                                                             tCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAg                                                                            >  1:459612/1‑80 (MQ=255) tCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAg                                                                            >  2:459595/1‑80 (MQ=255)  ccGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAgg                                                                           >  2:459604/1‑80 (MQ=255)         aCCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGtt                                                                   <  2:459563/81‑1 (MQ=255)                 aaTCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGt                                                           <  2:459564/81‑1 (MQ=255)                    ccTCGCCTTAACCGGCTGCGATCGACTAACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAGAAAGTTTCGCCAGTgg                                                         >  2:459614/1‑80 (MQ=255)                        gCCTTAACCGGCTGCGATCGACTGACTATCTCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACg                                                     >  1:459606/1‑80 (MQ=255)                        gCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTAc                                                      <  2:459565/79‑1 (MQ=255)                        gCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACg                                                     <  1:459566/80‑1 (MQ=255)                        gCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACg                                                     >  2:459622/1‑80 (MQ=255)                              aCCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAAtt                                               >  1:459598/1‑80 (MQ=255)                                  gCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATcc                                          >  2:459603/1‑81 (MQ=255)                                       gATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCt                                     >  2:459615/1‑81 (MQ=255)                                       gATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCt                                     >  1:459619/1‑81 (MQ=255)                                           gactgactATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTtct                                  <  2:459567/80‑1 (MQ=255)                                             ctgactATCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAg                               <  1:459568/81‑1 (MQ=255)                                                   aTCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTc                         >  1:459593/1‑81 (MQ=255)                                                    tCGCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTc                         <  1:459569/80‑1 (MQ=255)                                                      gCACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCa                      >  2:459609/1‑81 (MQ=255)                                                       cACCGAATTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCAc                     >  2:459624/1‑81 (MQ=255)                                                             aTTTACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAg               >  2:459608/1‑81 (MQ=255)                                                              tttACTGAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGc              <  1:459570/81‑1 (MQ=255)                                                                    gAAGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCAt        >  1:459616/1‑81 (MQ=255)                                                                      aGGAGCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGa      <  2:459571/81‑1 (MQ=255)                                                                          gCTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGa  <  1:459572/81‑1 (MQ=255)                                                                           cTGTAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCg   >  1:459634/1‑79 (MQ=255)                                                                              |                                                                             TCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGA  >  NC_000913/2579342‑2579495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑